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Western blot - Kallikrein 2 antibody (ab77253)
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Product Name
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Kallikrein 2 antibody
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See all Kallikrein 2 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Kallikrein 2
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Immunogen
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Synthetic peptide: KHQSLRPDEDSSHD (Human) from the internal region of the protein sequence according to NP_005542.1; NP_001002231.1.
BLAST 'KHQSLRPDEDSSHD' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is expected to recognize both reported Kallikrein 2 isoforms (NP_005542.1; NP_001002231.1).
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1/16000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 29 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human prostate lysate
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Relevance
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Kallikreins (KLKs) are a subgroup of trypsin-like serine proteases and are implicated in carcinogenesis. Kallikrein 2 is most closely related to Kallikrein 3 (PSA), with 78% identity and with kallikrein 1, at 68% identity. Like Kallikrein 3, Kallikrein 2 is found in greatest abundance in the prostate, but at much lower levels than Kallikrein 3. In addition, Kallikrein 2 seems to be more labile than Kallikrein 1 or Kallikrein 3 and is often found in a cleaved form. Kallikrein 2 is inhibited by Protein C Inhibitor (PCI).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Kallikrein 2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Kallikrein 2 antibody images, references, products related to ab77253 and other tools.
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Kallikrein 2 antibody images:
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Search PubMed (MEDLINE) for references to Kallikrein 2
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Kallikrein 2 antibody - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Western blot - Kallikrein 2 antibody (ab77253)