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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Overview
Product name
Anti-Kallikrein 2 antibody
See all Kallikrein 2 products (6) ...
Description
Goat polyclonal to Kallikrein 2
Specificity
This antibody is expected to recognize both reported Kallikrein 2 isoforms (NP_005542.1; NP_001002231.1).
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: KHQSLRPDEDSSHD (Human) from the internal region of the protein sequence according to NP_005542.1; NP_001002231.1.
KHQSLRPDED SSHD
Positive control
Human prostate lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Western blot - Kallikrein 2 antibody (ab77253)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Antibody detection limit dilution 1/16000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 29 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Target information above from: UniProt accessionP20151
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hK2 antibody
- KLK2A 2 antibody
- KLK2A2 antibody
see all
Database links
- Entrez Gene: 3817 Human
- Omim: 147960 Human
- SwissProt: P20151 Human
- Unigene: 515560 Human
Anti-Kallikrein 2 antibody images:
Western blot - Kallikrein 2 antibody (ab77253)
Anti-Kallikrein 2 antibody (ab77253) at 0.1 µg/ml + human prostate lysate in RIPA buffer at 35 µg
Predicted band size : 29 kDa
Observed band size : 22 kDa (why is the actual band size different from the predicted?)
References for Anti-Kallikrein 2 antibody (ab77253)
ab77253 has not yet been referenced specifically in any publications.
Publishing research using ab77253? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"