Kallikrein 4 antibody (ab71234)

Product Name 

Kallikrein 4 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Kallikrein 4

Immunogen 

Synthetic peptide from the center region of human Kallikrein 4 conjugated to KLH

Reacts with 
(species key)

Hu, Ms

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse brain tissue lysate.

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins

Relevance 

The human tissue Kallikrein gene family encodes 15 serine proteases. All Kallikreins share structural similarities including cysteine residues, a catalytic triad of His, Asp, and Ser residues, typically five coding exons and varied intron phases. Kallikreins are predominantly secreted as inactive zymogens prior to activation by cleavage of an N terminal peptide and all function extracellularly. Kallikreins can be activated autocatalytically, via other Kallikreins, or additional proteases. While structurally similar, Kallikrein family members have distinct functions and have key roles in many physiological and pathological processes. Many human tissue Kallikreins also show promise as cancer biomarkers, which may facilitate earlier detection and characterization of many forms of cancer.

Kallikrein 4 (hK4) was first described as enamel matrix serine proteinase 1, a serine protease involved in degradation of amelogenin during tooth development and tooth enamel turnover. Unlike another enamel processing enzyme, MMP20, hK4 is not restricted to dental tissues and has been identified in a range of normal and cancerous tissues and cell lines. Androgen response elements have been identified in hK4 genes and the hK4 levels have been shown to be modified by androgens and estrogens, similar to hK3 (PSA). hK4 has also been shown to activate pro-uPa by generating the two chain form after cleavage of the one chain form and thus may participate in an enzyme activation cascade. The Kallikrein 4 signal sequence is thought to be insufficient to allow secretion of hK4 and several reports indicate a predominately nuclear isolation.
The hK4 structure differs from the Kallikrein 1-3 in having a shorter "kallikrein loop", the sequence thought to impart specificity to the classical kallikreins. Endogenous inhibitors include kallistatin, protein C inhibitor and alpha 1 proteinase inhibitor, although hK4 can be found complexed to a number of different proteinase inhibitors.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Ammonium Sulphate Precipitation

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

ab71234 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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