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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Overview
Product name
Anti-Kallikrein 5 antibody - Catalytic domain
See all Kallikrein 5 products (5) ...
Description
Rabbit polyclonal to Kallikrein 5 - Catalytic domain
Specificity
This antibody is specific for Kallikrein 5, it does not react with the other Kallikreins (1-4, 6-15).
Tested applications
WB, SDS-PAGEmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the catalytic domain of human Kallikrein 5. (Peptide available as ab43087.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Western blot - Kallikrein 5 antibody - Catalytic domain (ab40951)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
SDS-PAGE
Application notes
SDS-PAGE: Use at an assay dependent dilution. Processed Kallikrein 5 has an apparent mass of 32-34 kDa on reduced SDS PAGE gels. WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 32 kDa. Dilution optimised using Chromogenic detection. A range of different sized bands can be seen for Kallikrein 5 in different tissues and in cell culture media, ranging from 16-32 kDa and likely representing different proteolytically processed forms, but perhaps also the different splice variants. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Kallikrein 5 was originally described from the skin as an enzyme involved in turnover of the stratum corneum, and later cloned from human EST libraries as a Kallikrein like enzyme. The skin is known to contain trypsin like and chymotrypsin like proteinases of 32 kDa and 25 kDa respectively, and these were determined to be hK5 and hK7. Kallikrein 5 is found is greatest abundance in the skin, and also in the brain, with lesser signal in the kidney, lung, mammary gland, and testis. Human breast milk is reported to contain high levels of Kallikrein 5. Less stringent PCR detects hK5 in many tissues, and analysis of cell lines by Western blot shows that many cell lines make hK5.
Cellular localization
Secreted
Alternative names
- HK5 antibody
- Kallikrein 5 antibody
- Kallikrein L2 antibody
see all
Database links
- Entrez Gene: 25818 Human
- Omim: 605643 Human
- SwissProt: Q9Y337 Human
- Unigene: 50915 Human
Anti-Kallikrein 5 antibody - Catalytic domain images:
Western blot - Kallikrein 5 antibody - Catalytic domain (ab40951)
All lanes : Anti-Kallikrein 5 antibody - Catalytic domain (ab40951) at 1/1000 dilution
Lane 1 : Kallikrein 5 at 0.05 µg
Lane 2 : Kallikrein 5 at 0.01 µg
Lane 3 : Kallikrein 5 at 0.001 µg
Predicted band size : 32 kDa
Observed band size : 32-38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 66 kDa (possible dimer).
Protocols
References for Anti-Kallikrein 5 antibody - Catalytic domain (ab40951)
ab40951 has not yet been referenced specifically in any publications.
Publishing research using ab40951? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"