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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Overview
Product name
Anti-Kallikrein 5 antibody - Kallikrein loop
See all Kallikrein 5 products (5) ...
Description
Rabbit polyclonal to Kallikrein 5 - Kallikrein loop
Specificity
This antibody reacts with Kallikrein 5, but does not react with the other Kallikreins (1-7 and 9-15).
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the Kallikrein loop area of human Kallikrein 5.
(Peptide available as ab41423.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Western blot - Kallikrein 5 antibody - Kallikrein loop (ab28565)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Predicted molecular weight: 32 kDa.(1/1000 (when using colorimetric substrates such as BCIP/NBT)- 1/5000 (chemiluminescent substrates). Dilution optimised using Chromogenic detection.)
ICC/IF: Use at an assay dependent dilution. (PubMed: 21163944)
Target
Relevance
Kallikrein 5 was originally described from the skin as an enzyme involved in turnover of the stratum corneum, and later cloned from human EST libraries as a Kallikrein like enzyme. The skin is known to contain trypsin like and chymotrypsin like proteinases of 32 kDa and 25 kDa respectively, and these were determined to be hK5 and hK7. Kallikrein 5 is found is greatest abundance in the skin, and also in the brain, with lesser signal in the kidney, lung, mammary gland, and testis. Human breast milk is reported to contain high levels of Kallikrein 5. Less stringent PCR detects hK5 in many tissues, and analysis of cell lines by Western blot shows that many cell lines make hK5.
Cellular localization
Secreted
Alternative names
- HK5 antibody
- Kallikrein 5 antibody
- Kallikrein 5 antibody
see all
Database links
- Entrez Gene: 25818 Human
- Omim: 605643 Human
- SwissProt: Q9Y337 Human
- Unigene: 50915 Human
Anti-Kallikrein 5 antibody - Kallikrein loop images:
Western blot - Kallikrein 5 antibody - Kallikrein loop (ab28565)
All lanes : Anti-Kallikrein 5 antibody - Kallikrein loop (ab28565) at 1/1000 dilution
Lane 1 : Kallikrein 5 at 0.05 µg
Lane 2 : Kallikrein 5 at 0.01 µg
Lane 3 : Kallikrein 5 at 0.001 µg
Predicted band size : 32 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-Kallikrein 5 antibody - Kallikrein loop (ab28565)
This product has been referenced in:
- Jiang Ret al. Kallikrein-5 promotes cleavage of desmoglein-1 and loss of cell-cell cohesion in oral squamous cell carcinoma. J Biol Chem 286:9127-35 (2011). ICC/IF; Human.Read more (PubMed: 21163944) »
- Ghosh Set al. Urinary-type plasminogen activator receptor/alpha 3 beta 1 integrin signaling, altered gene expression, and oral tumor progression. Mol Cancer Res 8:145-58 (2010). IHC-P; Mouse.Read more (PubMed: 20145038) »
See all 2 publications for this product
Publishing research using ab28565? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"