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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Overview
Product name
Anti-Kallikrein 5 antibody
See all Kallikrein 5 products (5) ...
Description
Goat polyclonal to Kallikrein 5
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Rat, Dog, Chimpanzee, Zebrafish
Immunogen
Synthetic peptide: KAGRDSCQGD, corresponding to internal sequence amino acids 235-244 of Human Kallikrein 5
KAGRDSCQGD
Positive control
Human Prostate lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Western blot - Kallikrein 5 antibody (ab60173)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab60173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).
Target
Relevance
Kallikrein 5 was originally described from the skin as an enzyme involved in turnover of the stratum corneum, and later cloned from human EST libraries as a Kallikrein like enzyme. The skin is known to contain trypsin like and chymotrypsin like proteinases of 32 kDa and 25 kDa respectively, and these were determined to be hK5 and hK7. Kallikrein 5 is found is greatest abundance in the skin, and also in the brain, with lesser signal in the kidney, lung, mammary gland, and testis. Human breast milk is reported to contain high levels of Kallikrein 5. Less stringent PCR detects hK5 in many tissues, and analysis of cell lines by Western blot shows that many cell lines make hK5.
Cellular localization
Secreted
Alternative names
- HK5 antibody
- Kallikrein 5 antibody
- Kallikrein L2 antibody
see all
Database links
- Entrez Gene: 25818 Human
- Entrez Gene: 408211 Rat
- Omim: 605643 Human
- SwissProt: Q9Y337 Human
- Unigene: 50915 Human
Anti-Kallikrein 5 antibody images:
Western blot - Kallikrein 5 antibody (ab60173)
ab60173 antibody (0.1µg/ml) staining of Kallikrein 5 in Human prostate lysate (35µg protein in RIPA buffer)
Predicted band size : 32 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-Kallikrein 5 antibody (ab60173)
ab60173 has not yet been referenced specifically in any publications.
Publishing research using ab60173? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"