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Anti-Kallikrein 6 antibody - Kallikrein loop (ab28301)

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Overview

Product name

Anti-Kallikrein 6 antibody - Kallikrein loop
See all Kallikrein 6 products (5) ...

Description

Rabbit polyclonal to Kallikrein 6 - Kallikrein loop

Specificity

The antibody reacts with human Kallikrein 6, and does not react with the human kallikreins (hK1-15).

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide based on the Kallikrein loop area of human Kallikrein 6.

(Peptide available as ab41384.)

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Kallikrein 6 antibody - Kallikrein loop (ab28301)Western blot - Kallikrein 6 antibody - Kallikrein loop (ab28301) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. Predicted molecular weight: 27 kDa.(1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. A range of different sized bands can be seen for Kallikrein 6 in different tissues and in cell culture media, ranging from 16-32 kDa and likely representing different proteolytically processed forms, but perhaps also the different splice variants. Dilution optimised using Chromogenic detection.)

Target

Function

Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.

Tissue specificity

In fluids, highest levels found in milk of lactating women followed by cerebrospinal fluid, nipple aspirate fluid and breast cyst fluid. Also found in serum, seminal plasma and some amniotic fluids and breast tumor cytosolic extracts. Not detected in urine. At the tissue level, highest concentrations found in glandular tissues such as salivary glands followed by lung, colon, fallopian tube, placenta, breast, pituitary and kidney. Not detected in skin, spleen, bone, thyroid, heart, ureter, liver, muscle, endometrium, testis, pancreas, seminal vesicle, ovary, adrenals and prostate. In brain, detected in gray matter neurons (at protein level). Colocalizes with pathological inclusions such as Lewy bodies and glial cytoplasmic inclusions. Overexpressed in primary breast tumors but not expressed in metastatic tumors.

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.

Post-translational
modifications

Inactivated by autolytic cleavage after Arg-80.

Cellular localization

Secreted. Nucleus > nucleolus. Cytoplasm. Mitochondrion. Microsome. In brain, detected in the nucleus of glial cells and in the nucleus and cytoplasm of neurons. Detected in the mitochondrial and microsomal fractions of HEK-293 cells and released into the cytoplasm following cell stress.

Target information above from: UniProt accessionQ92876 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Bssp antibody
  • hK 6 antibody
  • hK6 antibody
  • Kallikrein 6 precursor antibody
  • Kallikrein related peptidase 6 antibody
  • Kallikrein-6 antibody
  • Kallikrein6 antibody
  • KLK 6 antibody
  • Klk 7 antibody
  • KLK 9 antibody
  • Klk6 antibody
  • KLK6_HUMAN antibody
  • Klk7 antibody
  • KLK9 antibody
  • MGC9355 antibody
  • mGK 1 antibody
  • mGK1 antibody
  • Neurosin antibody
  • Protease M antibody
  • Protease serine 18 antibody
  • Protease serine 9 antibody
  • PRSS 18 antibody
  • PRSS 9 antibody
  • PRSS18 antibody
  • PRSS9 antibody
  • Serine protease 18 antibody
  • Serine protease 9 antibody
  • SP 59 antibody
  • SP59 antibody
  • Tissue kallikrein antibody
  • TK antibody
  • Zyme antibody
see all

Anti-Kallikrein 6 antibody - Kallikrein loop images:

  Western blot - Kallikrein 6 antibody - Kallikrein loop (ab28301)

Western blot - Kallikrein 6 antibody - Kallikrein loop (ab28301)

All lanes : Anti-Kallikrein 6 antibody - Kallikrein loop (ab28301) at 1/1000 dilution

Lane 1 : Cell line A2058. SF
Lane 2 : Cell line A2058. TPA
Lane 3 : Cell line A2058. IL1-alpha
Lane 4 : Cell line A2058. TNF-alpha
Lane 5 : Cell line Hs 294T. SF
Lane 6 : Cell line Hs 294T. TPA
Lane 7 : Cell line Hs 294T. IL1-beta
Lane 8 : Cell line Hs 294T. TNF-alpha
Lane 9 : Cell line DU145. SF
Lane 10 : Cell line DU145. TPA
Lane 11 : Cell line DU145. IL1-beta
Lane 12 : Cell line LNCap. SF
Lane 13 : Cell line LNCap. Testosterone

Lysates/proteins at 0.01 µg per lane.


Predicted band size : 27 kDa
Observed band size : 27 kDa
Additional bands at : 97 kDa (possible post-translational modification).

References for Anti-Kallikrein 6 antibody - Kallikrein loop (ab28301)

ab28301 has not yet been referenced specifically in any publications.

Publishing research using ab28301? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"