Kallikrein 6 antibody - Kallikrein loop (ab28301)

Product Name 

Kallikrein 6 antibody - Kallikrein loop

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Kallikrein 6 - Kallikrein loop

Immunogen 

Synthetic peptide based on the Kallikrein loop area of human Kallikrein 6.

(Peptide available as ab41384.)

Reacts with 
(species key)

Hu

Specificity 

The antibody reacts with human Kallikrein 6, and does not react with the human kallikreins (hK1-15).

We have a range of domain specific antibodies for this target. For a full list please see all Kallikrein 6 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at an assay dependent dilution. Predicted molecular weight: 27 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. A range of different sized bands can be seen for Kallikrein 6 in different tissues and in cell culture media, ranging from 16-32 kDa and likely representing different proteolytically processed forms, but perhaps also the different splice variants. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic, Mitochondrial, Nuclear and Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other

Relevance 

Kallikrein 6, also known as Zyme, Neurosin, PRSS9, myelencephalon-specific protease (MSP) and protease M, is a trypsin-like serine proteinase. Kallikrein 6 was originally described from the brain as an enzyme involved in degradation of amyloid plaque protein (APP) and hK6 was thought to be a beta secretase. Kallikrein 6 was shown to be elevated in the sera patients with Alzheimer’s disease and Parkinson’s disease and in animal models of multiple sclerosis. The neuronal protein alpha-synuclein was shown to be cleaved by hK6, as has APP and hK6 has been implicated in vascular morphogenesis and angiogenesis. MSP has also been shown to rapidly degrade myelin-specific protein, laminin and fibronectin. Kallikrein 6 is found in greatest abundance in the brain and neuronal tissues, with lesser levels in the kidney, thymus, spleen and lymph nodes. Human breast milk is reported to contain high levels of Kallikrein 6 and hK6 has been purified from cerebral spinal fluid (CSF). Less stringent PCR detects hK6 in many tissues and analysis of cell lines by Western blot shows that many cell lines make hK6. Endogenous inhibitors include kallistatin, protein-C inhibitor and 1-proteinase inhibitor, although Kallikrein 6 can be found complexed to a number of different proteinase inhibitors.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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