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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Overview
Product name
Anti-Kallikrein 6 antibody - Kallikrein loop
See all Kallikrein 6 products (5) ...
Description
Rabbit polyclonal to Kallikrein 6 - Kallikrein loop
Specificity
The antibody reacts with human Kallikrein 6, and does not react with the human kallikreins (hK1-15).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the Kallikrein loop area of human Kallikrein 6.
(Peptide available as ab41384.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Tags & Cell Markers >> Cell Type Markers >> Epi / Endo-thelial
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
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Western blot - Kallikrein 6 antibody - Kallikrein loop (ab28301)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 27 kDa.(1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. A range of different sized bands can be seen for Kallikrein 6 in different tissues and in cell culture media, ranging from 16-32 kDa and likely representing different proteolytically processed forms, but perhaps also the different splice variants. Dilution optimised using Chromogenic detection.)
Target
Function
Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.
Tissue specificity
In fluids, highest levels found in milk of lactating women followed by cerebrospinal fluid, nipple aspirate fluid and breast cyst fluid. Also found in serum, seminal plasma and some amniotic fluids and breast tumor cytosolic extracts. Not detected in urine. At the tissue level, highest concentrations found in glandular tissues such as salivary glands followed by lung, colon, fallopian tube, placenta, breast, pituitary and kidney. Not detected in skin, spleen, bone, thyroid, heart, ureter, liver, muscle, endometrium, testis, pancreas, seminal vesicle, ovary, adrenals and prostate. In brain, detected in gray matter neurons (at protein level). Colocalizes with pathological inclusions such as Lewy bodies and glial cytoplasmic inclusions. Overexpressed in primary breast tumors but not expressed in metastatic tumors.
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Post-translational
modifications
Inactivated by autolytic cleavage after Arg-80.
Cellular localization
Secreted. Nucleus > nucleolus. Cytoplasm. Mitochondrion. Microsome. In brain, detected in the nucleus of glial cells and in the nucleus and cytoplasm of neurons. Detected in the mitochondrial and microsomal fractions of HEK-293 cells and released into the cytoplasm following cell stress.
Target information above from: UniProt accessionQ92876
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bssp antibody
- hK 6 antibody
- hK6 antibody
see all
Database links
- Entrez Gene: 5653 Human
- Omim: 602652 Human
- SwissProt: Q92876 Human
- Unigene: 79361 Human
Anti-Kallikrein 6 antibody - Kallikrein loop images:
Western blot - Kallikrein 6 antibody - Kallikrein loop (ab28301)
All lanes : Anti-Kallikrein 6 antibody - Kallikrein loop (ab28301) at 1/1000 dilution
Lane 1 : Cell line A2058.
SF
Lane 2 : Cell line A2058. TPA
Lane 3 : Cell line A2058. IL1-alpha
Lane 4 : Cell line A2058. TNF-alpha
Lane 5 : Cell line Hs 294T. SF
Lane 6 : Cell line Hs 294T. TPA
Lane 7 : Cell line Hs 294T. IL1-beta
Lane 8 : Cell line Hs 294T. TNF-alpha
Lane 9 : Cell line DU145.
SF
Lane 10 : Cell line DU145.
TPA
Lane 11 : Cell line DU145. IL1-beta
Lane 12 : Cell line LNCap.
SF
Lane 13 : Cell line LNCap.
Testosterone
Lysates/proteins at 0.01 µg per lane.
Predicted band size : 27 kDa
Observed band size : 27 kDa
Additional bands at : 97 kDa (possible post-translational modification).
Protocols
References for Anti-Kallikrein 6 antibody - Kallikrein loop (ab28301)
ab28301 has not yet been referenced specifically in any publications.
Publishing research using ab28301? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"