Kallikrein 8 antibody - Catalytic domain (ab40954)

Product Name 

Kallikrein 8 antibody - Catalytic domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Kallikrein 8 - Catalytic domain

Immunogen 

Synthetic peptide based on the catalytic domain of Human Kallikrein 8.

(Peptide available as ab43090.)

Reacts with 
(species key)

Hu

Specificity 

Ab 40954 reacts with human Kallikrein 8 and does not react with the other human kallikreins (hK1-7, 9-15).

We have a range of domain specific antibodies for this target. For a full list please see all Kallikrein 8 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 (when using colorimetric substrates such as BCIP/NBT) - 1/5000 (for chemiluminescent substrates). Predicted molecular weight: 33 kDa. A number of different splice variants and partial sequences have been reported for Kallikrein 8, the longest encodes a protein of 305 amino acids with a predicted mass of 32.97 kDa. The 260 amino acid form has a predicted mass of 28 kDa. The 164, 134 and 119 amino acid versions have a predicted mass of 17.9, 14.7 and 12.7 kDa respectively but it is unclear if these proteins are produced. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins

Relevance 

Kallikrein 8 (hK8), also known as neuropsin, brain serine protease 1 (BSP1), tumor associated differentially expressed gene 14 (TADG14), PRSS19, HNP and ovasin, is a trypsin like serine protease. Originally described from mouse brain, KLK8 was implicated in neural plasticity and learning. Kallikrein 8 is thought to be involved in neurite outgrowth and fasciculation during nervous system development. Kallikrein 8 is induced in neurite injury, and Kallikrein 8 has been shown to be elevated in the hippocampus on Alzheimer’s patients’ brains. Animal models of autoimmune encephalomyelitis (EAE) show elevated Kallikrein 8 production. Kallikrein 8 null mice have aberrant synapses in their hippocampus, compared to wild type mice, and are predisposed to seizures. Kallikrein 8 is thought to regulate the early phase of long-term potentiation (LTP), possibly by cleaving fibronectin and other substrates. Kallikrein 8 is also found in the uterus, where it is hormonally regulated. Cervical cancer cell lines express high levels of Kallikrein 8, and it may be useful as a tumor marker. Kallikrein 8 is not limited to the brain and uterus, as many tissues and cell lines make the protein. Endogenous inhibitors include serine protease inhibitor 3 and protein C inhibitor, although Kallikrein 8 can be found complexed to a number of different protease inhibitors. A number of different splice variants and partial sequences have been reported for Kallikrein 8, the longest encodes a protein of 305 amino acids with a predicted mass of 32.97 kDa and a pI of 8.59. The 260 amino acid form has a predicted mass of 28 kDa and pI of 8.18, the 164, 134 and 119 amino acid versions 17.9, 14.7 and 12.7 kDa respectively, but it is unclear if these proteins are produced.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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