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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Overview
Product name
Anti-Kallikrein 9 antibody - Catalytic domain
See all Kallikrein 9 products (4) ...
Description
Rabbit polyclonal to Kallikrein 9 - Catalytic domain
Specificity
Ab40955 reacts with human Kallikrein 9 and does not react with the other human kallikreins (hK1-8, 10-15).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the catalytic domain of Human Kallikrein 9.
(Peptide available as ab43091.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Western blot - Kallikrein 9 antibody - Catalytic domain (ab40955)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 (when using colorimetric substrates such as BCIP/NBT) - 1/5000 (for chemiluminescent substrates). Predicted molecular weight: 36 kDa. Glycosylation and other post-translational modifications make the Kallikrein 9 protein run more slowly on SDS PAGE gels, usually around 36 kDa. A range of different sized bands can be seen for Kallikrein 9 in different tissues and in cell culture media, ranging from 16-30 kDa and likely representing different proteolytically processed forms. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Tissue specificity
Skin, thymus, trachea, cerebellum and spinal cord.
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ9UKQ9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Kallikrein 8 antibody
- Kallikrein 9 antibody
- Kallikrein L3 antibody
see all
Database links
- Entrez Gene: 284366 Human
- Omim: 605504 Human
- SwissProt: Q2XQG6 Human
- SwissProt: Q6QA55 Human
- SwissProt: Q9HC80 Human
- SwissProt: Q9UKQ9 Human
- Unigene: 448942 Human
- Unigene: 5193 Human
Anti-Kallikrein 9 antibody - Catalytic domain images:
Western blot - Kallikrein 9 antibody - Catalytic domain (ab40955)
All lanes : Anti-Kallikrein 9 antibody - Catalytic domain (ab40955) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from A2058 human melanoma cancer cells (treated with SF)
Lane 2 : Whole cell lysate prepared from A2058 human melanoma cancer cells (treated with TPA)
Lane 3 : Whole cell lysate prepared from A2058 human melanoma cancer cells (treated with IL1-alpha)
Lane 4 : Whole cell lysate prepared from A2058 human melanoma cancer cells (treated with TNF-alpha)
Lysates/proteins at 5 µl per lane.
Predicted band size : 36 kDa
Protocols
References for Anti-Kallikrein 9 antibody - Catalytic domain (ab40955)
ab40955 has not yet been referenced specifically in any publications.
Publishing research using ab40955? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"