Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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ab41387 |
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All lanes : Anti-Kallikrein 9 antibody - Kallikrein loop (ab28566) at 1/1000 dilution
Lane 1 : Cell line MDA-MB-231. SF at 0.01 µg
Lane 2 : Cell line MDA-MB-231. 100nM beta-Estradiol
Lane 3 : Cell line MDA-MB-231. 100nM Progesterone
Lane 4 : Cell line Hs294T. SF at 0.01 µg
Lane 5 : Cell line Hs294T. TPA at 0.01 µg
Lane 6 : Cell line Hs294T. IL1-alpha at 0.01 µg
Lane 7 : Cell line Hs294T. IL1-beta at 0.01 µg
Lane 8 : Cell line Hs294T. TNF-alpha at 0.01 µg
Lane 9 : Cell line HT 1080. 10nM beta-Estradiol
Lane 10 : Cell line HT 1080. 100nM beta-Estradiol
Lane 11 : Cell line HT 1080. 10nM Progesterone
Lane 12 : Cell line HT 1080. 100nM Progesterone
Predicted band size : 30 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible post-translational modification),96 kDa (possible post-translational modification).
Glycosylation and other post-translational modifications make the Kallikrein-9 protein run more slowly on SDS PAGE gels, usually around 36 kDa.
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