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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab28566 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Kallikrein 9 antibody - Kallikrein loop (ab28566)
All lanes : Anti-Kallikrein 9 antibody - Kallikrein loop (ab28566) at 1/1000 dilution
Lane 1 : Cell line MDA-MB-231. SF at 0.01 µg
Lane 2 : Cell line MDA-MB-231. 100nM beta-Estradiol
Lane 3 : Cell line MDA-MB-231. 100nM Progesterone
Lane 4 : Cell line Hs294T. SF at 0.01 µg
Lane 5 : Cell line Hs294T. TPA at 0.01 µg
Lane 6 : Cell line Hs294T. IL1-alpha at 0.01 µg
Lane 7 : Cell line Hs294T. IL1-beta at 0.01 µg
Lane 8 : Cell line Hs294T. TNF-alpha at 0.01 µg
Lane 9 : Cell line HT 1080. 10nM beta-Estradiol
Lane 10 : Cell line HT 1080. 100nM beta-Estradiol
Lane 11 : Cell line HT 1080. 10nM Progesterone
Lane 12 : Cell line HT 1080. 100nM Progesterone
Predicted band size : 30 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible post-translational modification),96 kDa (possible post-translational modification).
Glycosylation and other post-translational modifications make the Kallikrein-9 protein run more slowly on SDS PAGE gels, usually around 36 kDa.
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