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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Overview
Product name
Anti-Kallikrein 9 antibody - Kallikrein loop
See all Kallikrein 9 products (4) ...
Description
Rabbit polyclonal to Kallikrein 9 - Kallikrein loop
Specificity
ab28566 reacts with human Kallikrein-9, and does not react with the other human kallikreins (hK1-15).
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide based on the Kallikrein loop area of Human Kallikrein 9.
(Peptide available as ab41387.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified and then concentrated.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Western blot - Kallikrein 9 antibody - Kallikrein loop (ab28566)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates - 1/5000 for chemiluminescent substrates. Detects a range of bands of approximately 16-36 kDa representing different proteolytically processed forms (predicted molecular weight: 30 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Tissue specificity
Skin, thymus, trachea, cerebellum and spinal cord.
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ9UKQ9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Kallikrein 8 antibody
- Kallikrein 9 antibody
- Kallikrein 9 antibody
see all
Database links
- Entrez Gene: 284366 Human
- Entrez Gene: 101533 Mouse
- Omim: 605504 Human
- SwissProt: Q2XQG6 Human
- SwissProt: Q6QA55 Human
- SwissProt: Q9HC80 Human
- SwissProt: Q9UKQ9 Human
- SwissProt: P15949 Mouse
see all
Anti-Kallikrein 9 antibody - Kallikrein loop images:
Western blot - Kallikrein 9 antibody - Kallikrein loop (ab28566)
All lanes : Anti-Kallikrein 9 antibody - Kallikrein loop (ab28566) at 1/1000 dilution
Lane 1 : Cell line MDA-MB-231. SF at 0.01 µg
Lane 2 : Cell line MDA-MB-231. 100nM beta-Estradiol
Lane 3 : Cell line MDA-MB-231. 100nM Progesterone
Lane 4 : Cell line Hs294T. SF at 0.01 µg
Lane 5 : Cell line Hs294T. TPA at 0.01 µg
Lane 6 : Cell line Hs294T. IL1-alpha at 0.01 µg
Lane 7 : Cell line Hs294T. IL1-beta at 0.01 µg
Lane 8 : Cell line Hs294T. TNF-alpha at 0.01 µg
Lane 9 : Cell line HT 1080. 10nM beta-Estradiol
Lane 10 : Cell line HT 1080. 100nM beta-Estradiol
Lane 11 : Cell line HT 1080. 10nM Progesterone
Lane 12 : Cell line HT 1080. 100nM Progesterone
Predicted band size : 30 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa (possible post-translational modification),96 kDa (possible post-translational modification).
Glycosylation and other post-translational modifications make the Kallikrein-9 protein run more slowly on SDS PAGE gels, usually around 36 kDa.
Protocols
References for Anti-Kallikrein 9 antibody - Kallikrein loop (ab28566)
ab28566 has not yet been referenced specifically in any publications.
Publishing research using ab28566? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"