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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Overview
Product name
Anti-Kallikrein 9 antibody
See all Kallikrein 9 products (4) ...
Description
Rabbit polyclonal to Kallikrein 9
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide, corresponding to amino acids 239-250 of Human KLK9.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
This antibody recognizes the gene product hk9 of human KLK9 (also known as KLK-L3), one of the many kallikrein serine proteases associated with carcinogenesis. KLK9 is expressed in thymus, testis, spinal cord, salivary gland, ovary and skin. Like other kallikreins, KLK9 is regulated by steroid hormones in cancer cells.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
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Immunocytochemistry/ Immunofluorescence-Kallikrein 9 antibody(ab7284)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab7284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 28 kDa.
ICC/IF: Use a concentration of 1 µg/ml
Target
Tissue specificity
Skin, thymus, trachea, cerebellum and spinal cord.
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ9UKQ9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Kallikrein 8 antibody
- Kallikrein 9 antibody
- Kallikrein L3 antibody
see all
Database links
- Entrez Gene: 284366 Human
- Omim: 605504 Human
- SwissProt: Q2XQG6 Human
- SwissProt: Q6QA55 Human
- SwissProt: Q9HC80 Human
- SwissProt: Q9UKQ9 Human
- Unigene: 448942 Human
- Unigene: 5193 Human
Anti-Kallikrein 9 antibody images:
Immunocytochemistry/ Immunofluorescence-Kallikrein 9 antibody(ab7284)
ICC/IF image of ab7284 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7284, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Kallikrein 9 antibody (ab7284)
ab7284 has not yet been referenced specifically in any publications.
Publishing research using ab7284? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"