|
|
|
|
Immunocytochemistry/ Immunofluorescence-Kallikrein 9 antibody(ab7284)
(enlarge)
|
|
|
|
|
Product Name
|
Kallikrein 9 antibody
|
| |
See all Kallikrein 9 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Kallikrein 9
|
|
Immunogen
|
Synthetic peptide, corresponding to amino acids 239-250 of Human KLK9.
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ICC/IF, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 28 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Secreted
|
|
Research areas
|
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
|
|
Relevance
|
Kallikrein 9, also known as Kallikrein-Like 3 (KLK-L3), is a chymotrypsin-like serine proteinase. Kallikrein 9 was discovered as the locus for kallikreins on chromosome 19 was more fully mapped and found by similarity to the other tissue kallikreins. Kallikrein 9 has been found in the ovary, thymus, testis, prostate, skin, breast and neuronal tissues and is made by many cell lines in culture. Kallikrein 9 levels in breast cancer and uterine cancer patients have been reported to drop as the disease progresses, thus hK9 might be considered a favorable prognostic marker. Different splice variants of hK9 have been reported, although it is not yet known if they produce functional proteins. The full length Kallikrein 9 encodes for a 250 amino acid protein, with a predicted mass of 27.5 kDa and a pI of 7.53. The 234 amino acid form predicts a protein of 26 kDa with a pI of 9.76 and this quite basic pI might give the shorter form a very different function or localization. The shorter sequence also diverges before the catalytic serine residue, making it unlikely to be proteolytically active. Pre-pro-kallikrein 9 has the 17 amino acid signal sequence is removed before secretion, and the Pro-kallikrein 9 is activated to Kallikrein 9 by removal of the 5 amino acid propeptide domain.
This antibody recognizes the gene product hk9 of human KLK9 (also known as KLK-L3), one of the many kallikrein serine proteases associated with carcinogenesis. KLK9 is expressed in thymus, testis, spinal cord, salivary gland, ovary and skin. Like other kallikreins, KLK9 is regulated by steroid hormones in cancer cells.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Kallikrein 9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Kallikrein 9 antibody images, references, products related to ab7284 and other tools.
|
| |
Kallikrein 9 antibody images:
|
 Immunocytochemistry/ Immunofluorescence-Kallikrein 9 antibody(ab7284)
ICC/IF image of ab7284 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7284, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Kallikrein 9
|
| |
Kallikrein 9 antibody - more information
|
Customer reviews (feedback) regarding Kallikrein 9 antibody |
Customer FAQs regarding Kallikrein 9 antibody |
Protocols for Kallikrein 9 antibody |
Price and availability of products related to Kallikrein 9 antibody |
| |
Kallikrein 9 antibody - related products:
|
|
|
| |
Kallikrein 9 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Kallikrein 9 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
