Products:Immunology >> Immunoglobulins >> Light Chain >> Kappa
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which dilution do you use for mem09 used in WB??? always 1ug/ml |
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ANSWER: |
Since the immunoglobulin concentration of this product is 1mg/ml, I would suggest a starting concentration of 1ug/ml. Adjust this accordingly from the results obtained.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-Kappa light chain antibody [MEM-09] (ab1050) at 1/2000 dilution + Human B lymphocytes, whole cell lysate at 15 µg
Secondary
Alkaline phosphatase conjugated goat anti-mouse antibody at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 11 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
The blot was blocked with 2% milk for 2 hours at 22°C and incubated with the primary antibody for 1 hour and 20 minutes at 22°C.
This image is courtesy of an anonymous Abreview
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