Anti-Keap1 antibody [OTI1B4] (ab119403)
Key features and details
- Mouse monoclonal [OTI1B4] to Keap1
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Keap1 antibody [OTI1B4]
See all Keap1 primary antibodies -
Description
Mouse monoclonal [OTI1B4] to Keap1 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant full length Human Keap1 produced in HEK293T cells (NP_987096).
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Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA; HEK293T cells transfected with pCMV6-ENTRY Keap1 overexpress plasmid; Jurkat and HeLa cells; Human endometrium, endometrium adenocarcinoma and bladder carcinoma tissues.
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General notes
Dilute in PBS (pH7.3) before use.
This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab119403 was purified from TCS by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI1B4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB | (3) |
1/500 - 1/2000. Predicted molecular weight: 70 kDa.
|
IHC-P | (1) |
1/150.
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Notes |
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Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 70 kDa. |
IHC-P
1/150. |
Target
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Function
Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. -
Tissue specificity
Broadly expressed, with highest levels in skeletal muscle. -
Sequence similarities
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. -
Domain
The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5. -
Post-translational
modificationsUbiquitinated and subject to proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9817 Human
- Entrez Gene: 50868 Mouse
- Omim: 606016 Human
- SwissProt: Q14145 Human
- SwissProt: Q9Z2X8 Mouse
- Unigene: 465870 Human
- Unigene: 248266 Mouse
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Alternative names
- Cytosolic inhibitor of Nrf2 antibody
- INrf 2 antibody
- INrf2 antibody
see all
Images
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All lanes : Anti-Keap1 antibody [OTI1B4] (ab119403) at 1/500 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 70 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189) -
All lanes : Anti-Keap1 antibody [OTI1B4] (ab119403) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HeLa cell extract
Lane 3 : SVT2 cell extract
Lane 4 : A549 cell extract
Lane 5 : COS7 cell extract
Lane 6 : Jurkat cell extract
Lane 7 : MDCK cell extract
Lane 8 : PC12 cell extract
Lane 9 : MCF7 cell extract
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 70 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189) -
ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
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ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
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ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
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ab119403 at 1/100 dilution staining Keap1 in HEK293T cells transfected with either pCMV6-ENTRY Keap1 overexpress plasmid (Red) or empty vector control plasmid (Blue) and then analysed by Flow Cytometry (Intracellular).
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ab119403 at 1/100 dilution staining Keap1 in HeLa cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
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ab119403 at 1/100 dilution staining Keap1 in Jurkat cells by Flow cytometry (Intracellular) (Red) compared to a nonspecific negative control antibody (Blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab119403 has been referenced in 25 publications.
- Chen Y et al. Bushen Culuan Decoction Ameliorates Premature Ovarian Insufficiency by Acting on the Nrf2/ARE Signaling Pathway to Alleviate Oxidative Stress. Front Pharmacol 13:857932 (2022). PubMed: 35462905
- Gao J et al. Oxymatrine inhibits the pyroptosis in rat insulinoma cells by affecting nuclear factor kappa B and nuclear factor (erythroid-derived 2)-like 2 protein/heme oxygenase-1 pathways. Korean J Physiol Pharmacol 26:165-174 (2022). PubMed: 35477544
- Wu L et al. Vitamin C Attenuates Oxidative Stress, Inflammation, and Apoptosis Induced by Acute Hypoxia through the Nrf2/Keap1 Signaling Pathway in Gibel Carp (Carassius gibelio). Antioxidants (Basel) 11:N/A (2022). PubMed: 35624798
- Zhang YY et al. Pretreatment of nucleus pulposus mesenchymal stem cells with appropriate concentration of H2O2 enhances their ability to treat intervertebral disc degeneration. Stem Cell Res Ther 13:340 (2022). PubMed: 35883157
- Chen Y et al. Preventive Electroacupuncture Alleviates Oxidative Stress and Inflammation via Keap1/Nrf2/HO-1 Pathway in Rats with Cyclophosphamide-Induced Premature Ovarian Insufficiency. Biomed Res Int 2022:6718592 (2022). PubMed: 36060148