Overview

  • Product name
  • Description
    Goat polyclonal to Keap1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide:

    EVTPSQHGNRTFS

    with a Cysteine residue linker, corresponding to internal sequence amino acids 41-53 of Human Keap1 (NP_036421.2).

  • Positive control
    • HeLa and NIH3T3 cell lysates.
  • General notes
    Reported variants represent identical protein: NP_036421.2, NP_987096.1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab118285 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
  • Tissue specificity
    Broadly expressed, with highest levels in skeletal muscle.
  • Sequence similarities
    Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • Domain
    The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
  • Post-translational
    modifications
    Ubiquitinated and subject to proteasomal degradation.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytosolic inhibitor of Nrf2 antibody
    • INrf 2 antibody
    • INrf2 antibody
    • Keap 1 antibody
    • KEAP1 antibody
    • KEAP1_HUMAN antibody
    • Kelch like ECH associated protein 1 antibody
    • Kelch like family member 19 antibody
    • Kelch like protein 19 antibody
    • Kelch-like ECH-associated protein 1 antibody
    • Kelch-like protein 19 antibody
    • KIAA0132 antibody
    • KLHL 19 antibody
    • KLHL19 antibody
    • MGC10630 antibody
    • MGC1114 antibody
    • MGC20887 antibody
    • MGC4407 antibody
    • MGC9454 antibody
    see all

Images

  • Anti-Keap1 antibody (ab118285) at 0.2 µg/ml + NIH3T3 cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 70 kDa
  • ab118285 (3.8µg/ml) staining of paraffin embedded Human Cerebellum following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows textured cytoplasm staining in Purkinje cell and processes.

References

ab118285 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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