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Product Name
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Keap1 antibody
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See all Keap1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Keap1
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Note on antibody grade |
Fast-Track antibodies
- Immunogen affinity purified
- high ELISA titre values
- released early (to accelerate your research)
- 950 Abpoints to the 1st researcher
who submits conclusive data (positive or negative):
Read the Fast-Track antibody Terms of Use
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Keap1. (Peptide available as ab32770.)
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Predicted to react
(species key)
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Hu, Ms, Rat, Cow, Pig
(Due to sequence homology)
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Abreviews
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Application notes
(see key)
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Recommended dilutions
This antibody gave a positive result in ELISA against the immunizing peptide (ab32770).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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Keap1 is a zinc metalloprotein that represses Nrf2 and acts as a sensor of xenobiotic and oxidative stresses. Keap1 has also been implicated as having a role in cancer - the Nrf2-Keap1 system contributes to protection against various pathologies, including carcinogenesis, liver toxicity, respiratory distress and inflammation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 0.40 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Keap1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Keap1 antibody images, references, products related to ab31973 and other tools.
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Keap1 antibody images:
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This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Please read the definition of 'Fast-Track antibody'
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 Immunocytochemistry/ Immunofluorescence - Keap1 antibody (ab31973)
ICC/IF image of ab31973 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab31973, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HepG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
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Search PubMed (MEDLINE) for references to Keap1
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Keap1 antibody - more information
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Customer reviews (feedback) regarding Keap1 antibody |
Customer FAQs regarding Keap1 antibody |
Protocols for Keap1 antibody |
Price and availability of products related to Keap1 antibody |
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Keap1 antibody - related products:
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Keap1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Keap1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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