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Overview
Product name
Anti-Keap1 antibody
See all Keap1 products (5) ...
Description
Rabbit polyclonal to Keap1
Tested applications
ELISA, WB, ICC/IFmore details
Cross reactivity
Predicted to work with
Mouse, Rat, Cow, Human, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Keap1.
(Peptide available as ab32770.)
Positive control
This antibody gave a positive result in Hek293, HepG2, NIH3T3 whole cell lysates and Rat Testis.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. (: This antibody gave a positive result in ELISA against the immunizing peptide (ab32770).)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).(Please refer to abreview for further experimental details.)
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
Tissue specificity
Broadly expressed, with highest levels in skeletal muscle.
Sequence similarities
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.
Domain
The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
Post-translational
modifications
Ubiquitinated and subject to proteasomal degradation.
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
Target information above from: UniProt accessionQ14145
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cytosolic inhibitor of Nrf2 antibody
- INrf 2 antibody
- INrf2 antibody
see all
Database links
- Entrez Gene: 532791 Cow
- Entrez Gene: 9817 Human
- Entrez Gene: 50868 Mouse
- Entrez Gene: 100136900 Pig
- Entrez Gene: 117519 Rat
- Omim: 606016 Human
- SwissProt: Q14145 Human
- SwissProt: Q9BPY9 Human
see all
Anti-Keap1 antibody images:
Western blot - Anti-Keap1 antibody (ab31973)
All lanes : Anti-Keap1 antibody (ab31973) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 4 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 70 kDa
Observed band size : 70 kDa
Additional bands at : 17 kDa,50 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Keap1 antibody (ab31973)
ICC/IF image of ab31973 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab31973, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HepG2 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
References for Anti-Keap1 antibody (ab31973)
ab31973 has not yet been referenced specifically in any publications.
Publishing research using ab31973? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"