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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab66620? Please let us know so that we can cite the reference in this datasheet
ab66620 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Keap1 antibody (ab66620)
ab66620, at 4 - 8 µg/ml, staining Keap1 in human kidney tissue by immunohistochemistry on paraffin-embedded tissue. The arrows indicate positively stained epithelial cells of the renal tubule.
Western blot - Keap1 antibody (ab66620)
Lane 1 : Anti-Keap1 antibody (ab66620) at 5 µg/ml
Lane 2 : Anti-Keap1 antibody (ab66620) at 2.5 µg/ml
Lane 3 : Anti-Keap1 antibody (ab66620) at 1.25 µg/ml
Lane 1 : Cell lysate prepared form HepG2 cells
Lane 2 : Cell lysate prepared form HepG2 cells
Lane 3 : Cell lysate prepared form HepG2 cells
Lysates/proteins at 25 µg/ml per lane.
Predicted band size : 70 kDa
Western blot - Keap1 antibody (ab66620)
Anti-Keap1 antibody (ab66620) at 1 µg/ml + Lysate prepared from human 293T cells at 10 µg
Secondary
IRDye® 700DX-conjugated donkey polyclonal to rabbit IgG at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 70 kDa
Observed band size : 70 kDa
Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
This image is a courtesy of Anonymous Abreview
Immunocytochemistry/ Immunofluorescence-Keap1 antibody(ab66620)
ICC/IF image of ab66620 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66620, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Keap1 antibody for WB in Human (66620)
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