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Overview
Product name
Anti-Keap1 antibody
See all Keap1 products (5) ...
Description
Rabbit polyclonal to Keap1
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Dog, Human
Predicted to work with
Cow, Pig, Zebrafish
Immunogen
Synthetic peptide: ETWTFVAPMK HRRSALGITV HQGRIYVLGG YDGHTFLDSV ECYDPDTDTW , corresponding to amino acids 542-591 of Human Keap1
ETWTFVAPMK HRRSALGITV HQGRIYVLGG YDGHTFLDSV ECYDPDTDTW
Positive control
HepG2 cell lysate. Human kidney tissue.
General notes
Expression of Keap1 shuttles between cytoplasm and nucleus.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1.0 µg/mlDetects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Function
Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
Tissue specificity
Broadly expressed, with highest levels in skeletal muscle.
Sequence similarities
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.
Domain
The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
Post-translational
modifications
Ubiquitinated and subject to proteasomal degradation.
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
Target information above from: UniProt accessionQ14145
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cytosolic inhibitor of Nrf2 antibody
- INrf 2 antibody
- INrf2 antibody
see all
Database links
- Entrez Gene: 532791 Cow
- Entrez Gene: 476713 Dog
- Entrez Gene: 9817 Human
- Entrez Gene: 50868 Mouse
- Entrez Gene: 100136900 Pig
- Entrez Gene: 117519 Rat
- Entrez Gene: 321837 Zebrafish
- Omim: 606016 Human
see all
Anti-Keap1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Keap1 antibody (ab66620)
ab66620, at 4 - 8 µg/ml, staining Keap1 in human kidney tissue by immunohistochemistry on paraffin-embedded tissue. The arrows indicate positively stained epithelial cells of the renal tubule.
Western blot - Keap1 antibody (ab66620)
Lane 1 : Anti-Keap1 antibody (ab66620) at 5 µg/ml
Lane 2 : Anti-Keap1 antibody (ab66620) at 2.5 µg/ml
Lane 3 : Anti-Keap1 antibody (ab66620) at 1.25 µg/ml
Lane 1 : Cell lysate prepared form HepG2 cells
Lane 2 : Cell lysate prepared form HepG2 cells
Lane 3 : Cell lysate prepared form HepG2 cells
Lysates/proteins at 25 µg/ml per lane.
Predicted band size : 70 kDa
Western blot - Keap1 antibody (ab66620)
Anti-Keap1 antibody (ab66620) at 1 µg/ml + Lysate prepared from human 293T cells at 10 µg
Secondary
IRDye® 700DX-conjugated donkey polyclonal to rabbit IgG at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 70 kDa
Observed band size : 70 kDa
Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
This image is a courtesy of Anonymous Abreview
Immunocytochemistry/ Immunofluorescence-Keap1 antibody(ab66620)
ICC/IF image of ab66620 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Keap1 antibody (ab66620)
ab66620 has not yet been referenced specifically in any publications.
Publishing research using ab66620? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"