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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Keap1 antibody (ab66620)
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Western blot - Keap1 antibody (ab66620)
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Product Name
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Keap1 antibody
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See all Keap1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Keap1
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Immunogen
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Synthetic peptide: ETWTFVAPMK HRRSALGITV HQGRIYVLGG YDGHTFLDSV ECYDPDTDTW , corresponding to amino acids 542-591 of Human Keap1
BLAST 'ETWTFVAPMK HRRSALGITV HQGRIYVLGG YDGHTFLDSV ECYDPDTDTW ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, Dog
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.0 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ELISA titre using peptide based assay: 1/1562500.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate. Human kidney tissue.
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Cellular localization
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Cytoplasmic
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
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Relevance
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Keap1 is a zinc metalloprotein that represses Nrf2 and acts as a sensor of xenobiotic and oxidative stresses. Keap1 has also been implicated as having a role in cancer - the Nrf2-Keap1 system contributes to protection against various pathologies, including carcinogenesis, liver toxicity, respiratory distress and inflammation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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Batch dependent within range: 1.00 - 2.00 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Expression of Keap1 shuttles between cytoplasm and nucleus.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Keap1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Keap1 antibody images, references, products related to ab66620 and other tools.
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Keap1 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Keap1 antibody (ab66620)
ab66620, at 4 - 8 µg/ml, staining Keap1 in human kidney tissue by immunohistochemistry on paraffin-embedded tissue. The arrows indicate positively stained epithelial cells of the renal tubule.
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Western blot - Keap1 antibody (ab66620)
Lane 1 : Keap1 antibody (ab66620) at 5 µg/ml
Lane 2 : Keap1 antibody (ab66620) at 2.5 µg/ml
Lane 3 : Keap1 antibody (ab66620) at 1.25 µg/ml
Lane 1 : Cell lysate prepared form HepG2 cells
Lane 2 : Cell lysate prepared form HepG2 cells
Lane 3 : Cell lysate prepared form HepG2 cells
Lysates/proteins at 25 µg/ml per lane.
Predicted band size : 70 kDa
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Search PubMed (MEDLINE) for references to Keap1
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Keap1 antibody - more information
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Customer reviews (feedback) regarding Keap1 antibody |
Customer FAQs regarding Keap1 antibody |
Protocols for Keap1 antibody |
Price and availability of products related to Keap1 antibody |
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Keap1 antibody - related products:
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Keap1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Keap1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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