Ken and Barbie antibody (ab21780)

Product Name 

Ken and Barbie antibody

Product type 

Primary antibodies

Description 

Mouse polyclonal to Ken and Barbie

Immunogen 

Fusion protein: WKTTDSAYLS SKCGGLRDRA DRRKQQYTSP QNLEPDQTLK YEVDSVDESR NAADFSSAFN SNDNCESAAE CERSGGHNNK EEDEDDCTHK DNKSDKDTDE corresponding to amino acids 121/220 of Drosophila melanogaster Ken and barbie.
BLAST 'WKTTDSAYLS SKCGGLRDRA DRRKQQYTSP QNLEPDQTLK YEVDSVDESR NAADFSSAFN SNDNCESAAE CERSGGHNNK EEDEDDCTHK DNKSDKDTDE ' with or

Reacts with 
(species key)

Dm

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 67 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Other factors

Relevance 

Mutations in the ken and barbie locus are accompanied by the malformation of terminalia in adult Drosophila. Male and female genitalia often remain inside the body, and the same portions of genitalia and analia are missing in a fraction of homozygous flies. Rotated and/or duplicated terminalia are also observed. Terminalia phenotypes are enhanced by mutations in the gap gene tailless, the homeobox gene caudal, and the decapentaplegic gene that encodes a TGFbeta-like morphogen. The ken and barbie gene encodes a protein with three CCHH-type zinc finger motifs that are conserved in several transcription factors such as Kruppel and BCL-6. All defects in ken and barbie mutants are fully rescued by the expression of a wild-type genomic construct, which establishes the causality between phenotypes and the gene.

Defects in ken result in low mating success and reduced copulation duration. Male and female genitalia often remain inside the body, and genitalia and analia are missing in some homozygous flies. These flies are named after the famous dolls who also lack these 'features'.


This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.

A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.

Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Database links 

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Constituents: 50% Glycerol

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Notes 

Produced from outbred CD1 mice

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