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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-Ken and Barbie antibody
Description
Mouse polyclonal to Ken and Barbie
Tested applications
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Immunogen
Fusion protein: WKTTDSAYLS SKCGGLRDRA DRRKQQYTSP QNLEPDQTLK YEVDSVDESR NAADFSSAFN SNDNCESAAE CERSGGHNNK EEDEDDCTHK DNKSDKDTDE, corresponding to amino acids 121/220 of Fruit fly (Drosophila melanogaster) Ken and Barbie
WKTTDSAYLS SKCGGLRDRA DRRKQQYTSP QNLEPDQTLK YEVDSVDESR NAADFSSAFN SNDNCESAAE CERSGGHNNK EEDEDDCTHK DNKSDKDTDE
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000. Predicted molecular weight: 67 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Relevance
Mutations in the ken and barbie locus are accompanied by the malformation of terminalia in adult Drosophila. Male and female genitalia often remain inside the body, and the same portions of genitalia and analia are missing in a fraction of homozygous flies. Rotated and/or duplicated terminalia are also observed. Terminalia phenotypes are enhanced by mutations in the gap gene tailless, the homeobox gene caudal, and the decapentaplegic gene that encodes a TGFbeta-like morphogen. The ken and barbie gene encodes a protein with three CCHH-type zinc finger motifs that are conserved in several transcription factors such as Kruppel and BCL-6. All defects in ken and barbie mutants are fully rescued by the expression of a wild-type genomic construct, which establishes the causality between phenotypes and the gene. Defects in ken result in low mating success and reduced copulation duration. Male and female genitalia often remain inside the body, and genitalia and analia are missing in some homozygous flies. These flies are named after the famous dolls who also lack these 'features'.
Cellular localization
Nuclear
Alternative names
- Ken & Barbie antibody
- ken and barbie antibody
- ken-and-barbie antibody
- Probable transcription factor Ken antibody
Database links
- Entrez Gene: 37785 Fruit fly (Drosophila melanogaster)
- SwissProt: O77459 Fruit fly (Drosophila melanogaster)
References for Anti-Ken and Barbie antibody (ab21780)
This product has been referenced in:
- Kugler SJet al. The Putzig-NURF nucleosome remodeling complex is required for ecdysone receptor signaling and innate immunity in Drosophila melanogaster. Genetics 188:127-39 (2011).Read more (PubMed: 21385730) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"