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Anti-Ki67 antibody [B126.1] - Proliferation Marker (ab8191)

Abreviews (2)Q&A (11)Specific References (9)

Overview

  • Product nameAnti-Ki67 antibody [B126.1] - Proliferation MarkerSee all Ki67 primary antibodies ...
  • Description
    Mouse monoclonal [B126.1] to Ki67 - Proliferation Marker
  • SpecificityThis antibody reacts with an epitope on a nuclear antigen Ki-67, which is expressed in cycling cells (G1, S, G2, Mphases). It is a marker of native Ki-67 and recombinant fragments of Ki-67. The specificity and reactivity of this clone is equal or superior to any other available Ki-67 monoclonal antibody. Another proliferation cell nuclear antibody[PCNA], ab29, produces far superior staining profiles than any of the Ki-67 monoclonals we have tested.
  • Tested applicationsFlow Cyt, IHC-Fr, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The recombinant peptide corresponding to a Ki-67 cDNA fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab8191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: Use at an assay dependent concentration.
IHC-Fr IHC-Fr: Use at an assay dependent dilution.
IHC-P IHC-P: 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. The antibody gave good results after 5 minutes of heat-mediated retrieval in a microwave oven.
ICC/IF ICC/IF: Use at an assay dependent concentration.

Target

  • FunctionThought to be required for maintaining cell proliferation.
  • Sequence similaritiesContains 1 FHA domain.
  • Developmental stageExpression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
  • Cellular localizationNucleus. Nucleus > nucleolus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.

    • Target information above from: UniProt accession P46013 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Antigen identified by monoclonal antibody Ki 67 antibodyAntigen KI-67 antibodyAntigen KI67 antibody
        KI67_HUMAN antibodyKIA antibodyMKI67 antibodyProliferation related Ki 67 antigen antibodyRP11-380J17.2 antibody
      see all

    Anti-Ki67 antibody [B126.1] - Proliferation Marker images

    • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Ki67 antibody [B126.1] - Proliferation Marker (ab8191)
      Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - Ki67 antibody [B126.1] - Proliferation Marker (ab8191)This image is courtesy of an Abreview submitted by Mrs Olga Bragina
      IHC-P image of ab8191 (1/50) staining human melanoma tumour sections. Secondary antibody: rabbit polyclonal to mouse IgG conjugated to HRP (ab6728).

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Ki67 antibody [B126.1] - Proliferation Marker (ab8191)
      Immunocytochemistry/ Immunofluorescence - Ki67 antibody [B126.1] - Proliferation Marker (ab8191)This image is courtesy of an anonymous Abreview
      ab8191 at 1/100 staining normal human primary fibroblasts by ICC/IF. The cells were formaldehyde fixed, permeabilized and fixed before incabation with the antibody for 2 hours at 4°C. An Alexa Fluor ® 488 conjugated donkey polycloanl was used as the secondary.

      See Abreview

    References for Anti-Ki67 antibody [B126.1] - Proliferation Marker (ab8191)

    This product has been referenced in:
    • Liu C  et al. Prickle1 is expressed in distinct cell populations of the central nervous system and contributes to neuronal morphogenesis. Hum Mol Genet N/A:N/A (2013). Mouse . Read more (PubMed: 23420014) »
    • Liu C  et al. Regulation of retinal progenitor expansion by Frizzled receptors: implications for microphthalmia and retinal coloboma. Hum Mol Genet 21:1848-60 (2012). IHC (Methanol fixed) ; Mouse . Read more (PubMed: 22228100) »

    See all 9 Publications for this product

    Publishing research using ab8191 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab8191 Submit a Question for ab8191

    Displaying 1 - 10 of 13 results for Abreviews and Q&A

    10
    Votes: Highest First

    Interested in trying this antibody with rat tissue.

    DISCOUNT CODE: ***
    Expiration date: December 22, 2012

    This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forab8191 with rat samplesand include this code in the “...

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    IHC-P and ICC/IF with human colon and human frozen cells: no signal
    other Ki67 antibody worked on same samples
    Ab: 1/50, 1/25, 1/100 1-4 h
    block: commercial protein block
    AR: pH 6, pH 8
    2nd: HRP or IF

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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    will test ab66155 and ab8191 in sELISA

    Thank you for your phone call.
    DISCOUNT CODE: xxx for ab66155
    DISCOUNT CODE: xxx for ab8191
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and testab66155 and ab8191 in sELISA. Each code will gi...

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    ab8191 is not giving any signal when used in IHC-Fr on human samples

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab49873 with the order number 972573. To check the status of the order p...

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    Thanks for your help. We appreciate your work. I hope we could soon receive a new antibody, we had already wasted a lot of time. We attach the receipt of Ki-67.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab8191 with the order number 962498. To check the status of the o...

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    We write all the answer and attach our protocol. We look forward to your reply. Thanks a lot! Order Details Antibody code: ab8191 Lot number: GR 5758-3 Purchase order number or preferably Abcam order number: Ge...

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    Thanks for providing us with your protocol and sample preparation methods. I agree that the vial of ab8191 you have received is not performing as expected. If you have purchased this antibody within the last 6 months or so I would be happy to offe...

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    Hi, We bought the primary antibody from your company before 2 weeks for IF (sample is glioma sample slide). We test it use different dilution (1:50; 1:100; 1:200), we use seconday antibody is donkey anti-mouse alexa fluor 594 (1:500; 1:1000), but t...

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    Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. In order to ...

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Ki67 antibody [B126.1] - Proliferation Marker

     Excellent
    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Normal Primary Fibroblast)
    Specification Normal Primary Fibroblast
    Fixative Formaldehyde
    Permeabilization Yes - TritonX100
    Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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    Verified customer

    Submitted Jul 24 2007

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ki67 antibody [B126.1] - Proliferation Marker

     Good
    Abreviews
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Melanoma tumors)
    Specification Melanoma tumors
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
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    Username

    Verified customer

    Submitted Aug 03 2006

    I am seeing neutrophil staining with this antibody. Was neutrophil staining seen in testing this antibody?

    Thank you for your enquiry. This antibody should not produce staining in neutrophils. The staining you see may be due to endogenous peroxidase present in neutrophils. Have you tried blocking endogenous peroxidase with H2O2? The protocol we recomme...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"