Overview

  • Product nameAnti-Ki67 antibody [EPR3610]
    See all Ki67 primary antibodies
  • Description
    Rabbit monoclonal [EPR3610] to Ki67
  • Tested applicationsIHC-Fr, WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues in Human Ki67.

  • Positive control
    • WB: HeLa cell lysates IHC-P: Human tonsil and colonic adenocarcinoma tissues ICC/IF: HeLa cells
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    This product is available conjugated to FITC see ab171753.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels.

    If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab92742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 359 kDa.
IHC-P 1/500 - 1/1000. Antigen retrieval is recommended.
Flow Cyt 1/100 - 1/550.
ICC/IF 1/250 - 1/500.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionThought to be required for maintaining cell proliferation.
    • Sequence similaritiesContains 1 FHA domain.
    • Developmental stageExpression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
    • Cellular localizationNucleus. Nucleus > nucleolus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • Antigen identified by monoclonal antibody Ki 67 antibody
      • Antigen KI-67 antibody
      • Antigen KI67 antibody
      • KI67_HUMAN antibody
      • KIA antibody
      • MKI67 antibody
      • Proliferation related Ki 67 antigen antibody
      • RP11-380J17.2 antibody
      see all

    Anti-Ki67 antibody [EPR3610] images

    • Overlay histogram showing Ramos cells stained with ab92742 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92742, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • ab92742 showing positive staining in Colonic adenocarcinoma tissue.

    • ab92742 showing positive staining in Normal colon tissue.

    • ab92742, at a 1/500 dilution, staining Ki67 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
    • ab92742, at a 1/500 dilution, staining Ki67 in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemistry.
    • ab92742, at a 1/250 dilution, staining Ki67 in HeLa cells by Immunofluorescence.
    • ab92742 showing positive staining in Ovarian carcinoma tissue.

    • ab92742 showing positive staining in Cervical carcinoma tissue.

    • ab92742 staining Ki67 in human tonsil tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized and blocked with 4% serum + 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 18 hours at 4°C. An Alexa Fluor® 568-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Magnification: 20X. Counterstained with DAPI.

      See Abreview

    • Anti-Ki67 antibody [EPR3610] (ab92742) at 1/500 dilution + HeLa lysates at 10 µg

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 359 kDa
      Observed band size : 395 kDa (why is the actual band size different from the predicted?)

    References for Anti-Ki67 antibody [EPR3610] (ab92742)

    This product has been referenced in:
    • Chen Z  et al. Clinicopathological significance of non-small cell lung cancer with high prevalence of Oct-4 tumor cells. J Exp Clin Cancer Res 31:10 (2012). IHC-P ; Human . Read more (PubMed: 22300949) »
    • Brassart-Pasco S  et al. Tetrastatin, the NC1 domain of the a4(IV) collagen chain: a novel potent anti-tumor matrikine. PLoS One 7:e29587 (2012). IHC-Fr . Read more (PubMed: 22539938) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample Human Cell (Breast cancer)
    Specification Breast cancer
    Permeabilization Yes - Triton X-100
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 09 2014

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 22°C
    Sample Human Tissue sections (Tonsil)
    Specification Tonsil
    Permeabilization Yes - 0.5%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 28 2014

    I tried to find the mouse and human specific skin cell markers, regrettably I couldn't find enough information about protein that are specific to human or mouse cells. I am sure further publication search will be helpful in answering this question. I w...

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    ab92742 has indeed been tested in western blot on mouse and rat samples and gave a negative result.

    Merci pour votre réponse.

    Il n'y à aucun problème. Envoyez nous les images quand vous êtes de retour au laboratoire.

    Je vous souhaite un bon été et je me réjouis d'avoir de vos nouvelles.

    J'ai reçu confirmation du laboratoire que l'anticorps avait été testé sur des cellules du cerveau, du cœur, des reins et de la rate de la souris (et rat) et ne donnait aucun signal. Le laboratoire n'avait pas test&eacu...

    Read More

    Merci beaucoup pour votre émail.

    Je suis désolée de ce malentendu et du délais que ceci à entrainé.

    Est-ce que vous pourriez m'envoyer des images du résultat? Je vais vérifier ...

    Read More

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