Alexa Fluor® 488 Anti-Ki67 antibody [KI67] (ab206633)
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [KI67] to Ki67
- Suitable for: ICC, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
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Overview
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Product name
Alexa Fluor® 488 Anti-Ki67 antibody [KI67]
See all Ki67 primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [KI67] to Ki67 -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Ki67. Nuclei of the Hodgkin lymphoma cell line L428.
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Positive control
- ICC/IF: U2OS cells. Flow Cyt (Intra): Human blood cells.
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General notes
This antibody is designed for intracellular flow cytometric analysis of human blood cells using 4 μl reagent per 100 μl of whole blood, or 106 cells in a suspension. The contents of a vial (0.4 ml) is sufficient for 100 tests.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
KI67 -
Isotype
IgG1 -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206633 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 1 µg/ml.
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Flow Cyt (Intra) |
Use 4µl for 106 cells.
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Notes |
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ICC
Use a concentration of 1 µg/ml. |
Flow Cyt (Intra)
Use 4µl for 106 cells. |
Target
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Function
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed. -
Sequence similarities
Contains 1 FHA domain.
Contains 16 K167R repeats.
Contains 1 PP1-binding domain. -
Developmental stage
Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815). -
Post-translational
modificationsPhosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. -
Cellular localization
Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106). - Information by UniProt
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Database links
- Entrez Gene: 4288 Human
- Omim: 176741 Human
- SwissProt: P46013 Human
- Unigene: 689823 Human
- Unigene: 80976 Human
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Alternative names
- Antigen identified by monoclonal antibody Ki 67 antibody
- Antigen identified by monoclonal antibody Ki-67 antibody
- Antigen KI-67 antibody
see all
Images
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Immunocytochemistry analysis of U2OS cells labelling Ki67 with ab206633 at 1 μg/mL. DAPI (blue) was used as a nuclear stain.
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Intracellular Flow Cytometry analysis of human peripheral blood mononuclear cells stimulated with PHA.Surface staining of CD25was followed by permeabilization and nuclear staining of Ki-67.
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Surface staining of PHA-stimulated (for 3 days) PBMC with anti-Ki-67 (Ki-67) Alexa Fluor 647.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab206633 has been referenced in 2 publications.
- Du F et al. E3 Ubiquitin Ligase Midline 1 Regulates Endothelial Cell ICAM-1 Expression and Neutrophil Adhesion in Abdominal Sepsis. Int J Mol Sci 24:N/A (2022). PubMed: 36614145
- Algaber A et al. Targeting FHL2-E-cadherin axis by miR-340-5p attenuates colon cancer cell migration and invasion. Oncol Lett 22:637 (2021). PubMed: 34295384