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Ki67 protein (Tagged-His Tag) (ab92248)

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Overview

  • Product nameKi67 protein (Tagged-His Tag)See all Ki67 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 2891-3256 of Human Ki67 with N terminal His tag; 366 amino acids, 57kDa (41.6kDa predicted from sequence). UniProt ID = P46013.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 134 µl distilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesRKLDAEDVIGSRRQPRAPKEKAQPLEDLASFQELSQTPGH TEELANGAADSFTS APKQTPDSGKPLKISRRVLRAPKV EPVGDVVSTRDPVKSQSKSNTSLPPLPFKR GGGKDGSV TGTKRLRCMPAPEEIVEELPASKKQRVAPRARGKSSEPVV IMKRSL RTSAKRIEPAEELNSNDMKTNKEEHKLQDSVP ENKGISLRSRRQNKTEAEQQIT EVFVLAERIEINRNEK KPMKTSPEMDIQNPDDGARKPIPRDKVTENKRCLRSAR QNESSQPKVAEESGGQKSAKVLMQNQKGKGEAGNSDSMCL RSRKTKSQPAASTL ESKSVQRVTRSVKRCAENPKKAED NVCVKKIRTRSHRDSEDI
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92248 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Antigen identified by monoclonal antibody Ki 67Antigen KI-67Antigen KI67
        KI67_HUMANKIAMKI67Proliferation related Ki 67 antigenRP11-380J17.2
      see all
  • FunctionThought to be required for maintaining cell proliferation.
  • Sequence similaritiesContains 1 FHA domain.
  • Developmental stageExpression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
  • Cellular localizationNucleus. Nucleus > nucleolus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.

    • Target information above from: UniProt accession P46013 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Ki67 protein (Tagged-His Tag) images

    • SDS-PAGE - Ki67 protein (Tagged-His Tag) (ab92248)
      SDS-PAGE - Ki67 protein (Tagged-His Tag) (ab92248)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Ki67 protein (Tagged-His Tag) (ab92248)

    ab92248 has not yet been referenced specifically in any publications.

    Publishing research using ab92248 ? Please let us know so that we can cite the reference in this datasheet

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    ELISA Abreview for Ki67 protein (Tagged-His Tag)

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    Abreviews
    Application ELISA

    Sample: Human Recombinant protein
    Blocking step: BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Type: Indirect

    Other product details
    Incubation time: 18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

    Primary antibody (in addition to 'Ki67 protein (Tagged-His Tag)')
    Primary antibody: Abcam primary antibody: Anti-Ki67 antibody (ab66155)
    Dilution: 1/10000

    Secondary antibody
    Name: Abcam antibody:Anti-Ki67 antibody [4A1]
    Dilution: 1/10000

    Additional data
    Additional Notes: Primary ab in this case was the detection (ab86373) and the capture was ab66155 used at 1/1000 and 1/100.
    Abcam response: This customer received an AbTrial discount to test this protein in ELISA, an untested application. The ELISA may have failed because the capture antibody ab66155 has not been tested in a sandwich assay (sELISA) with the detection antibody ab86373.
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    Verified customer

    Submitted Apr 23 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"