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Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)

Q&A (5)Specific References (1)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Sodium Deoxycholate, Tergitol-NP40, EDTA, Magnesium chloride, HEPES, Sucrose, Potassium chloride, Protease inhibitor, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notes Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab30203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References for Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)

This product has been referenced in:
  • Larsen MH  et al. Human leukocyte antigen-G in the male reproductive system and in seminal plasma. Mol Hum Reprod 17:727-38 (2011). WB . Read more (PubMed: 21813635) »

See 1 Publication for this product

Publishing research using ab30203 ? Please let us know so that we can cite the reference in this datasheet

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Submit a Question for ab30203

Displaying 1 - 5 of 5 results for Q&A

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Hello, I am preparing to use this lysate in a Western Blot as a positive control. I typically load 50ug of protein per sample. Can you tell me what protein concentration is recommended for this kidney lysate? Because it is a lysate, can I use less, 10ug p...

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Thank you for contacting Abcam regarding the use of this lysate in WB.


Regarding the amount of protein to load, this largely depends on the amount of specific protein you wish to detect present in the sample. For lysates, we typically reco...

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This antibody has high number or non specific bands even on positive control human kidney lysate. Multiple conditions were tested for primary antibody and secondary antibody dilutions with no improvements.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab76593.

To check the status of the order please contact our C...

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I am looking for kidney (human) normal tissue lysate. Specifically I would like a lysateofthe proximal tubule. Are these available?

Thank you for contacting us. I have had the opportunity to speak with the lab about your request. Unfortunately our lysates are not produced in a manner that would allow us to offer the proximal tubule as an isolate. We apologize for the inconvenience. I ...

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Could you supply donor information for each of these lysates? Is it possible to get lysates from different donors?

Thank you for your enquiry. The mouse lysates are from Balb/c. Usually the mouse lysates are from pooled donors, not from a single donor. If you ordered multile lots, you would receive different pooled donors in each lot. We may be able to provid...

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I have a query about a number of your Whole Cell Lysates. Can you tell me if ab30203(normal adult kidney whole cell lysate) is normal tissue from a diseased patient or normal tissue from a normal patient? Likewise for ab29231 and ab30051. Can you tell...

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Thanks for the inquiry. Ab30203 , ab29231 and ab30051 are all from normal patients. Ab30205 is from a clear cell carcinoma patient. Ab29745 is from a 42 week aged placenta. Please let me know if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"