General notesDiagnosis: Normal
Soluble protein fraction.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituents: 0.25% Sodium Deoxycholate, 0.1% SDS, PBS, 1µg/ml Pepstatin A, 1µg/ml Aprotinin, 1µg/ml Leupeptin, 1mM Sodium Orthovanadate, 1mM EDTA, 1mM PMSF, 1mM Sodium fluoride, pH 7.4
Please note that 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate, as a result the lysate may not be suitable for applications which require native protein in a non-denaturing buffer. The final Laemmli buffer concs are: 31.25mM Tris HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.005% Bromophenol Blue, 25mM TCEP.
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Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
Lysate notesThis product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction.
Please find below a range of products that relate to ab44816:
Extraction 1: soluble protein fraction
ab44820 Human Kidney Tumor Tissue lysate
ab44816 Human Kidney Normal Tissue lysate (matched)
Extraction 2: insoluble protein fraction
ab44827 Human Kidney Tumor Tissue lysate
ab44822 Human Kidney Normal Tissue lysate (matched)
References for Kidney (Human) Tissue Lysate - adult normal tissue (ab44816)
has not yet been referenced specifically in any publications.
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