Overview
- Product nameKidney (Rat) Whole Cell Lysate - normal tissueSee all Kidney lysates ...
- Tested applicationsWB more details
Properties
- FormLiquid
- Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
- Storage bufferPreservative: None
Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Sodium Deoxycholate, Tergitol-NP40, EDTA, Magnesium chloride, HEPES, Sucrose, Potassium chloride, Protease inhibitor, 0.15M Beta mercaptoethanol, pH 7.9 -
Concentration information loading... - Lysate notes Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab29480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use. |
References for Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
ab29480 has not yet been referenced specifically in any publications.
