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Kinesin 2 protein (Tagged-His Tag) (ab91696)

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Overview

  • Product nameKinesin 2 protein (Tagged-His Tag)See all KLC1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 301-578 of Human Kinesin 2, with an N terminal His tag; MWt 32kDa (UniProt Q07866).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 54 µl distilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAK QLNNLALLCQ NQGKYEEVEYYYQRALEIYQTKLGPDDP NVAKTKNNLASCYLKQGKFKQA ETLYKEILTRAHEREF GSVDDENKPIWMHAEEREECKGKQKDGTSFGEYG GWYK ACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSR KQGLDN VHKQRVAEVLNDPENMEKRRSRESLNVDVVKY ESGPDGGEEVSMSVEWNG DGTGSLKRSGSFSKLRASIR RSSEKLVR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91696 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Kinesin 2 60/70kDaKinesin light chain 1Kinesin2
        KLCKLC 1KLC1KLC1_HUMANKNS 2KNS 2AKNS2KNS2AMedulloblastoma antigen MU MB 2.50MGC15245
      see all
  • FunctionKinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
  • Tissue specificityFound in a variety of tissues. Mostly abundant in brain and spine.
  • Sequence similaritiesBelongs to the kinesin light chain family.
    Contains 6 TPR repeats.
  • Post-translational
    modifications    Isoform I is phosphorylated on Ser-600. Isoform J is phosphorylated on Ser-631.
  • Cellular localizationCytoplasm > cytoskeleton.

    • Target information above from: UniProt accession Q07866 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Kinesin 2 protein (Tagged-His Tag) images

    • SDS-PAGE - Kinesin 2 protein (Tagged-His Tag) (ab91696)
      SDS-PAGE - Kinesin 2 protein (Tagged-His Tag) (ab91696)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Kinesin 2 protein (Tagged-His Tag) (ab91696)

    ab91696 has not yet been referenced specifically in any publications.

    Publishing research using ab91696 ? Please let us know so that we can cite the reference in this datasheet

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