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Product name
Anti-Kinesin 5C antibody
See all Kinesin 5C products (2) ...
Description
Rabbit polyclonal to Kinesin 5C
Specificity
This antibody is specific for kinesin 5C and does not detect other kinesin isotypes.
Tested applications
IHC-P, ICC/IF, WB, IPmore details
Cross reactivity
Reacts with
Rat, Human
Does not react with
Mouse, Cow
Immunogen
Synthetic peptide: AVHAIRGGGGSSSNSTHYQK, corresponding to amino acids 938-957 of Human Kinesin 5C. (Peptide available as ab417 84.)
AVHAIRGGGGSSSNSTHYQK
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Kinesin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF: Use at an assay dependent dilution.
WB: Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IP: 1/4000.
Target
Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
Tissue specificity
Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary.
Sequence similarities
Belongs to the kinesin-like protein family. Kinesin subfamily.
Contains 1 kinesin-motor domain.
Domain
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionO60282
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ44735 antibody
- KIAA0531 antibody
- KIF 5C antibody
see all
Database links
- Entrez Gene: 3800 Human
- Omim: 604593 Human
- SwissProt: O60282 Human
- SwissProt: P56536 Rat
- Unigene: 6641 Human
- Unigene: 435557 Human
- Unigene: 660699 Human
- Unigene: 50834 Rat
see all
Anti-Kinesin 5C antibody images:
Western blot - Kinesin 5C antibody (ab5630)
Predicted band size : 110 kDa
Shows a Western blot of kinesin 5C on human retinal extract using ab5630. The expected band at 110 kDa is seen along with an unidentified band at 100 kDa.
Immunocytochemistry/ Immunofluorescence - Kinesin 5C antibody (ab5630)
ab5630 staining Kinesin 5C in COS-7 cells by Immunocytochemistry/ Immunofluorescence.GFP-Kif5c behaviour in living cells. Cropped still images from a time-lapse recording of GFP-Kif5c puncta (green) moving along a MT labelled with mCherry–-tubulin (red) in a live COS-7 cell. The GFP-Kif5c puncta move from right to left. Scale bar, 2 µm. The graph shows the respective velocity of the GFP-Kif5c puncta seen over time, demonstrating the rapid changes in velocity of those puncta.
Image from Dunn S et al, J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95. Epub 2008 Mar 11, Fig 2.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Kinesin 5C antibody(ab5630)
IHC image of ab5630 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5630, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Kinesin 5C antibody (ab5630)
This product has been referenced in:
- Dunn S et al. Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells. J Cell Sci 121:1085-95 (2008). ICC/IF; Monkey.Read more (PubMed: 18334549) »
- Smith MJ et al. Mapping the GRIF-1 binding domain of the kinesin, KIF5C, substantiates a role for GRIF-1 as an adaptor protein in the anterograde trafficking of cargoes. J Biol Chem 281:27216-28 (2006).Read more (PubMed: 16835241) »
See all 3 publications for this product
Publishing research using ab5630? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"