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Enriched fraction of porcine brain kinesin.
Our Abpromise guarantee covers the use of ab9097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.|
|ICC/IF||Use at an assay dependent dilution.|
This antibody gives typical dot-like staining characteristic for localization of kinesin in vesicles. Optimum staining of cell lines was observed on samples fixed with formaldehyde followed by Triton X-100 extraction.
Immunofluorescence staining of embryonal mouse fibroblasts 3T3 with MAb KN-01. Double-label fluorescence with polyclonal affinity-purified anti-tubulin antibody reveals a co-distribution of vesicles with microtubules on the cell periphery. Vesicles were stained with anti-Kinesin Heavy Chain MAb KN-01 (red), microtubules with anti-tubulin Ab (green) and nucleus with DAPI (blue).
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