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Western blot - Kinesin Heavy Chain antibody [KN-01] (ab9097)
![Western blot - Kinesin Heavy Chain antibody [KN-01] (ab9097)](/ps/datasheet/images/ab9097_1.gif)
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Immunocytochemistry/ Immunofluorescence - Kinesin Heavy Chain antibody [KN-01] (ab9097)
![Immunocytochemistry/ Immunofluorescence - Kinesin Heavy Chain antibody [KN-01] (ab9097)](/ps/datasheet/images/ab9097_2.gif)
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Product Name
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Kinesin Heavy Chain antibody [KN-01]
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See all Kinesin Heavy Chain antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [KN-01] to Kinesin Heavy Chain
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Immunogen
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Enriched fraction of porcine brain kinesin.
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Reacts with
(species key)
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Hu, Ms, Pig
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Specificity
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Reacts with conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. This antibody recognizes the heavy chain of kinesin.
No cross-reaction with the light chain of the molecule nor with other proteins was detected.
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution (see PMID: 16176937).
WB: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Neuroscience >> Cell Type Marker >> Neuron marker >> Axon marker
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Motor Proteins >> Kinesin
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules
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Relevance
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Kinesin is a microtubule associated force producing protein that may play a role in organelle transport. It is an oligomer composed of two heavy chains and two light chains. It interacts with GRIP1. It is composed of three structural domains: a large globular N terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. Found in newborn and adult brain, liver, kidney, spleen, heart, lung and sciatic nerve.
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Kinesin Heavy Chain antibody [KN-01] (ab9097) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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KN-01
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Isotype
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IgM
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Purity
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>95% by SDS-PAGE
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from ascites using gel filtration and precipitation methods.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Kinesin Heavy Chain antibody [KN-01] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Kinesin Heavy Chain antibody [KN-01] images, references, products related to ab9097 and other tools.
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Kinesin Heavy Chain antibody [KN-01] images:
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 Western blot - Kinesin Heavy Chain antibody [KN-01] (ab9097)
Reactivity of ab9097 with enriched preparation of porcine brain kinesin in Western Blotting. Total protein staining with Coomassie Blue (lane 1), immunostaining with anti-Kinesin Heavy Chain MAb ab9097(lane 2), immunostaining with anti-tubulin ab7749 (lane 3).
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Immunocytochemistry/ Immunofluorescence - Kinesin Heavy Chain antibody [KN-01] (ab9097)
This antibody gives typical dot-like staining characteristic for localization of kinesin in vesicles.
Optimum staining of cell lines was observed on samples fixed with formaldehyde
followed by Triton X-100 extraction. Immunofluorescence staining of embryonal mouse fibroblasts 3T3 with MAb KN-01.
Double-label fluorescence with polyclonal affinity-purified anti-tubulin antibody reveals a
co-distribution of vesicles with microtubules on the cell periphery.
Vesicles were stained with anti-Kinesin Heavy Chain MAb KN-01 (red), microtubules with anti-tubulin Ab
(green) and nucleus with DAPI (blue). Dr. E. Dráberová, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic.
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References for Kinesin Heavy Chain antibody [KN-01] (ab9097)
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This product has been used in: (two most recent references)
Argyropoulos G et al. KIF5B gene sequence variation and response of cardiac stroke volume to regular exercise. Physiol Genomics 36:79-88 (2009). PubMed: 18984674
Utton MA et al. Molecular motors implicated in the axonal transport of tau and alpha-synuclein. J Cell Sci 118:4645-54 (2005). PubMed: 16176937
See all 4 publication references for this product.
If you publish research using ab9097 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Kinesin Heavy Chain
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Kinesin Heavy Chain antibody [KN-01] - more information
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Kinesin Heavy Chain antibody [KN-01] - related products:
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Kinesin Heavy Chain antibody [KN-01] - other tools:
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Contact Abcam with a Technical Enquiry about Kinesin Heavy Chain antibody [KN-01] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Kinesin Heavy Chain antibody [KN-01] (ab9097)](/index.html?datasheet=9097#ab9097_1.gif)
![Immunocytochemistry/ Immunofluorescence - Kinesin Heavy Chain antibody [KN-01] (ab9097)](/index.html?datasheet=9097#ab9097_2.gif)
