Anti-Kinesin Heavy Chain antibody [KN-01] (ab9097)

Overview

  • Product nameAnti-Kinesin Heavy Chain antibody [KN-01]See all Kinesin Heavy Chain primary antibodies ...
  • Description
    Mouse monoclonal [KN-01] to Kinesin Heavy Chain
  • SpecificityWestern blot is not a tested application for this product. However if you would like to use the antibody for Western blotting, we suggest using non-reducing conditions and avoiding heat-mediated denaturation. Reacts with conventional kinesin associated with vesicles and with lower affinity with denaturated molecule. This antibody recognizes the heavy chain of kinesin. No cross-reaction with the light chain of the molecule nor with other proteins was detected.
  • Tested applicationsIHC-P, Flow Cyt, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    Enriched fraction of porcine brain kinesin.

Properties

Applications

Our Abpromise guarantee covers the use of ab9097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionMicrotubule-dependent motor required for normal distribution of mitochondria and lysosomes.
  • Sequence similaritiesBelongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • DomainComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localizationCytoplasm > cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
  • Target information above from: UniProt accession P33176 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Conventional kinesin heavy chain antibody
    • KIF 5B antibody
    • KIF5B antibody
    • Kinesin 1 antibody
    • Kinesin family member 5B antibody
    • Kinesin-1 heavy chain antibody
    • Kinesin1 antibody
    • KINH antibody
    • KINH_HUMAN antibody
    • KNS antibody
    • KNS 1 antibody
    • KNS1 antibody
    • Ubiquitous kinesin heavy chain antibody
    • UKHC antibody
    see all

Anti-Kinesin Heavy Chain antibody [KN-01] images

  • This antibody gives typical dot-like staining characteristic for localization of kinesin in vesicles. Optimum staining of cell lines was observed on samples fixed with formaldehyde followed by Triton X-100 extraction.

    Immunofluorescence staining of embryonal mouse fibroblasts 3T3 with MAb KN-01. Double-label fluorescence with polyclonal affinity-purified anti-tubulin antibody reveals a co-distribution of vesicles with microtubules on the cell periphery. Vesicles were stained with anti-Kinesin Heavy Chain MAb KN-01 (red), microtubules with anti-tubulin Ab (green) and nucleus with DAPI (blue).

  • ab9097 (4µg/ml) staining Kinesin Heavy Chain in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is very weak cytoplasmic staining seminal vesicle.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Overlay histogram showing HeLa cells stained with ab9097 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90970, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-Kinesin Heavy Chain antibody [KN-01] (ab9097)

This product has been referenced in:
  • Van Engelenburg SB & Palmer AE Imaging type-III secretion reveals dynamics and spatial segregation of Salmonella effectors. Nat Methods 7:325-30 (2010). ICC/IF . Read more (PubMed: 20228815) »
  • Argyropoulos G  et al. KIF5B gene sequence variation and response of cardiac stroke volume to regular exercise. Physiol Genomics 36:79-88 (2009). Read more (PubMed: 18984674) »

See all 5 Publications for this product

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I am sorry but the epitope mapping with recombinant humant kinesin is not finished yet.

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