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Overview

  • Product nameKininogen Human ELISA KitSee all Kininogen kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine
  • Assay typeSandwich
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.56 ng/ml - 100 ng/ml
  • Recovery

    94 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Kininogen is a plasma protein coagulation cofactor serving for the activation of zymogens prekallikrein, Factor XII and Factor XI and is also a substrate of each of their proteolytic forms. It circulates as a complex with these zymogens and links the plasma coagulation, fibrinolysis, complement activation, and blood pressure control. Kininogen is produced by the liver and weighs 120 kDa with 626 amino acids. Its plasma concentration ranges from 55 to 90 µg/ml and Kininogen exhibits anticoagulant properties and is a strong inhibitor of cysteine proteases. Upon cleavage by kallikrein, the released active peptide bradykinin mediates NO release, vasodilation, hypotension and pain. The remaining cleaved HK (HKa) exhibits antiadhesive and antiangiogenic activity, enhancing cell-associated fibrinolysis and releasing cytokines and chemokines to enhance inflammation. Patients with HK deficiency exhibit abnormal surface-mediated activation of fibrinolysis.

    ab108876 Kininogen Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Kininogen in urine, milk, saliva and cell culture supernatant. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Human Kininogen in less than 4 hours. A polyclonal antibody specific for Human Kininogen has been pre-coated onto a 96-well microplate with removable strips. Kininogen in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for Human Kininogen, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsELISA more details

Properties

  • Function(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
  • Tissue specificitySecreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
  • Involvement in diseaseDefects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
  • Sequence similaritiesContains 3 cystatin domains.
  • Post-translational
    modifications    Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracelllular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted > extracellular space.

    • Target information above from: UniProt accession P01042 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Alpha-2-thiol proteinase inhibitorFitzgerald factorHigh molecular weight kininogen
        HMWKIle-Ser-BradykininKallidin IKallidin IIKNG1KNG1_HUMANLow molecular weight growth-promoting factorWilliams-Fitzgerald-Flaujeac factor
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108876 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Kininogen Human ELISA Kit images

      • ELISA - Kininogen Human ELISA Kit - 1 x 96 Well Plate (ab108876)
        ELISA - Kininogen Human ELISA Kit - 1 x 96 Well Plate (ab108876)
        Standard curve

        The curve provided is for illustration only. A standard curve should be generated each time the assay is performed.

      Protocols

      References for Kininogen Human ELISA Kit (ab108876)

      ab108876 has not yet been referenced specifically in any publications.

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