Kininogen Human ELISA Kit (ab108876)
- Product nameKininogen Human ELISA KitSee all Kininogen kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine
- Assay typeSandwich
- Sensitivity= 1.5 ng/ml
- Range1.56 ng/ml - 100 ng/ml
- Assay time4h 00m
- Species reactivityReacts with: Human
- Product overview
Kininogen is a plasma protein coagulation cofactor serving for the activation of zymogens prekallikrein, Factor XII and Factor XI and is also a substrate of each of their proteolytic forms. It circulates as a complex with these zymogens and links the plasma coagulation, fibrinolysis, complement activation, and blood pressure control. Kininogen is produced by the liver and weighs 120 kDa with 626 amino acids. Its plasma concentration ranges from 55 to 90 µg/ml and Kininogen exhibits anticoagulant properties and is a strong inhibitor of cysteine proteases. Upon cleavage by kallikrein, the released active peptide bradykinin mediates NO release, vasodilation, hypotension and pain. The remaining cleaved HK (HKa) exhibits antiadhesive and antiangiogenic activity, enhancing cell-associated fibrinolysis and releasing cytokines and chemokines to enhance inflammation. Patients with HK deficiency exhibit abnormal surface-mediated activation of fibrinolysis.
ab108876 Kininogen Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of Human Kininogen in urine, milk, saliva and cell culture supernatant. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Human Kininogen in less than 4 hours. A polyclonal antibody specific for Human Kininogen has been pre-coated onto a 96-well microplate with removable strips. Kininogen in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for Human Kininogen, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Human Kininogen Antibody (50x) 1 x 140µl Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Kininogen coated Microplate 1 x 96 Well plate 1 unit Human Kininogen Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 2 x 15ml
- Research Areas
modificationsBradykinin is released from kininogen by plasma kallikrein.
Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
Phosphorylation sites are present in the extracelllular medium.
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
- Alpha-2-thiol proteinase inhibitorFitzgerald factorHigh molecular weight kininogen
- HMWKIle-Ser-BradykininKallidin IKallidin IIKNG1KNG1_HUMANLow molecular weight growth-promoting factorWilliams-Fitzgerald-Flaujeac factor
Our Abpromise guarantee covers the use of ab108876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Kininogen Human ELISA Kit images
The curve provided is for illustration only. A standard curve should be generated each time the assay is performed.
References for Kininogen Human ELISA Kit (ab108876)
ab108876 has not yet been referenced specifically in any publications.