Recombinant Anti-Kir2.1/KCNJ2 antibody [EPR4530] (ab109750)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4530] to Kir2.1/KCNJ2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Kir2.1/KCNJ2 antibody [EPR4530]
See all Kir2.1/KCNJ2 primary antibodies -
Description
Rabbit monoclonal [EPR4530] to Kir2.1/KCNJ2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- 293T and A549 cell lysates; Human brain tissue; SH SY5Y cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4530 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (1) |
1/100 - 1/250.
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
|
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
Notes |
---|
ICC/IF
1/100 - 1/250. |
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
-
Function
Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium. -
Tissue specificity
Heart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain. -
Involvement in disease
Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7) [MIM:170390]; also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features.
Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3) [MIM:609622]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves. -
Sequence similarities
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ2 subfamily. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 3759 Human
- Entrez Gene: 16518 Mouse
- Entrez Gene: 29712 Rat
- Omim: 600681 Human
- SwissProt: P63252 Human
- SwissProt: P35561 Mouse
- SwissProt: Q64273 Rat
- Unigene: 1547 Human
see all -
Alternative names
- Cardiac inward rectifier potassium channel antibody
- HHBIRK 1 antibody
- HHBIRK1 antibody
see all
Images
-
All lanes : Anti-Kir2.1/KCNJ2 antibody [EPR4530] (ab109750) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 48 kDa -
ab109750 at 1/250 dilution staining Kir2.1/KCNJ2 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-
ab109750 at 1/100 dilution staining Kir2.1/KCNJ2 in SH SY5Y cells by Immunofluorescence.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab109750 has been referenced in 8 publications.
- Wang YX et al. Elevated Kir2.1/nuclear N2ICD defines a highly malignant subtype of non-WNT/SHH medulloblastomas. Signal Transduct Target Ther 7:72 (2022). PubMed: 35273141
- Chen L et al. α1 -Adrenoceptors activate the NLRP3 inflammasome through downregulation of Kir2.1 in cardiac inflammation. Exp Physiol 107:589-600 (2022). PubMed: 35363405
- Cao N et al. Inhibition of KIR2.1 decreases pulmonary artery smooth muscle cell proliferation and migration. Int J Mol Med 50:N/A (2022). PubMed: 35856410
- Zhang JZ et al. A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles. Cell Stem Cell 24:802-811.e5 (2019). PubMed: 30880024
- Fancher IS et al. Hypercholesterolemia-Induced Loss of Flow-Induced Vasodilation and Lesion Formation in Apolipoprotein E-Deficient Mice Critically Depend on Inwardly Rectifying K+ Channels. J Am Heart Assoc 7:N/A (2018). WB ; Mouse . PubMed: 29502106
- Cho JH et al. Reverse electrical remodeling in rats with heart failure and preserved ejection fraction. JCI Insight 3:N/A (2018). WB ; Rat . PubMed: 30282820
- Utrilla RG et al. Kir2.1-Nav1.5 Channel Complexes Are Differently Regulated than Kir2.1 and Nav1.5 Channels Alone. Front Physiol 8:903 (2017). PubMed: 29184507
- Li X et al. Valsartan Upregulates Kir2.1 in Rats Suffering from Myocardial Infarction via Casein Kinase 2. Cardiovasc Drugs Ther 29:209-18 (2015). WB ; Rat . PubMed: 26095682