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Products:Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
Overview
Product name
Anti-Kir6.2 antibody
See all Kir6.2 products (6) ...
Description
Rabbit polyclonal to Kir6.2
Tested applications
ICC/IF, WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
A synthetic peptide from the C terminal region of human Kir6.2 conjugated to an immunogenic carrier protein
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cardiovascular >> Heart >> Cardiac arrhythmias
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
Signal Transduction >> Metabolism >> Energy Metabolism
Cardiovascular >> Hypoxia >> Associated Proteins
Neuroscience >> Neurotransmission >> Receptors / Channels >> Potassium Channels
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Immunocytochemistry/ Immunofluorescence-Kir6.2 antibody(ab81121)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab81121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: 1/300 - 1/2000.Predicted molecular weight: 44 kDa.(This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.)
IHC-P
IHC-P: 1/300 - 1/2000.IHC-P: 1/300 - 1/2000.
IHC-Fr
IHC-Fr: 1/300 - 1/2000.IHC-Fr: 1/300 - 1/2000.
Target
Relevance
ATP-sensitive potassium (K(ATP)) channels are found in endocrine cells, neurons and both smooth and striated muscle, where they play an important role in controlling insulin secretion and vascular tone, and protect neurons under metabolic stress. Kir6.2 is a member of the inward rectifier potassium channel family, which is characterised by a greater tendency to allow potassium flow into the cell rather than out of it. It associates with the sulphonylurea receptor SUR1/ABCC8 to form a subfamily of K(ATP) channels that, when mutated or misregulated, are associated with forms of hyperinsulinemic hypoglycemia, neonatal diabetes, or pre-disposition to type 2 diabetes mellitus.
Cellular localization
Cell Membrane; Multi-pass membrane protein
Alternative names
- ATP sensitive inward rectifier potassium channel 11 antibody
- Beta cell inward rectifier subunit antibody
- BIR antibody
see all
Database links
- Entrez Gene: 3767 Human
- Entrez Gene: 16514 Mouse
- Entrez Gene: 83535 Rat
- Omim: 600937 Human
- SwissProt: Q14654 Human
- SwissProt: Q61743 Mouse
- SwissProt: P70673 Rat
- Unigene: 248141 Human
see all
Anti-Kir6.2 antibody images:
Immunocytochemistry/ Immunofluorescence-Kir6.2 antibody(ab81121)
ICC/IF image of ab81121 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81121, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Kir6.2 antibody (ab81121)
ab81121 has not yet been referenced specifically in any publications.
Publishing research using ab81121? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"