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Klotho protein (Active) (ab84072)

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Overview

  • Product nameKlotho protein (Active)See all Klotho proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 34-549 of Human Klotho isoform 2; 516 amino acids, 65-70 kDa. Swiss ID = Q9UEF7.
  • Expression hostCHO

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab84072 is greater than 98% by SDS-PAGE gel and HPLC analyses.
  • Biological activityBiological Activity : Determined by the dose-dependent stimulation of the proliferation of murine NIH3T3 cells.
  • FormLyophilised:Reconstituted ab84072 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC. Avoid repeated freeze/thaw cycles.
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°CThis product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Additional notesEndotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Sequence notesEPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab84072 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use a concentration of 0.5 - 2 µg/ml.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab69208.

    • Protein info

    • Alternative names
        KLKLOT_HUMANKlotho peptide
  • FunctionMay have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
    The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
  • Tissue specificityPresent in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
  • Involvement in diseaseDefects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 1 family. Klotho subfamily.
  • DomainContains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.
  • Post-translational
    modifications    N-glycosylated.
  • Cellular localizationSecreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide.

    • Target information above from: UniProt accession Q9UEF7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Klotho protein (Active) (ab84072)

    ab84072 has not yet been referenced specifically in any publications.

    Publishing research using ab84072 ? Please let us know so that we can cite the reference in this datasheet

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    10
    Votes: Highest First

    Sehr geehrte Damen und Herren,

    Ich bin auf der Suche nach rekombinanten bzw natürlichem, aufgereinigten
    humanen alpha Klotho. Bieten Sie noch weitere Domänen/Versionen dieses
    Proteins an (130kDa Form; transmembrane Domän...

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    Vielen Dank für Ihre Anfrage.

    Leider ist das Klotho-Fragment der Aminosäuren 34-549 das einzige Klotho, das wir verfügbar haben. Unter ab84072 bieten wir die ungetagte Form an, unter ab124316 ist das gleiche Fragment mit einem C-t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"