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Klotho protein (Tagged-His Tag) (ab124316)

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Overview

  • Product nameKlotho protein (Tagged-His Tag)See all Klotho proteins and peptides ...
  • Protein descriptionRecombinant full length mature Human Klotho protein chain (amino acids 34-549) with a C terminal His tag (522 amino acids including tag); predicted mwt: 59.5 kDa (UniProt Q9UEF7 isoform 2).
  • Uniprot accessionQ9UEF7
  • Molecular weight59.500kDa inclusive of tags
  • Protein length516 amino acids
  • Expression hostCHO

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Purification notesab124316 was purifed using Ni-NTA chromatography. The product is filtered (0.4 µm) but not sterile, hence needs to be filtered by an appropriate sterile filter before using in cell culture.
  • E.C. Number3.2.1.31
  • FormLyophilised:Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Avoid storage of lyophilized product at room temperature. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term. Reconstituted protein can be stored at 4°C for a week.
  • Storage instructionsStore at -80°C
  • Storage bufferpH: 7.20
    Constituents: 99% Phosphate Buffer, 0.58% Sodium chloride
  • Concentration information loading...
  • Sequence notesEPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYHHHHH HH
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab124316 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution.
    ELISA ELISA: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent concentration.
  • Application notesThis peptide can be used with studies using ab69208.

    • Protein info

    • Alternative names
        KlKLOT_HUMANKlotho peptide
  • FunctionMay have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
    The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.
  • Tissue specificityPresent in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
  • Involvement in diseaseDefects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 1 family. Klotho subfamily.
  • DomainContains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.
  • Post-translational
    modifications    N-glycosylated.
  • Cellular localizationSecreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide.

    • Target information above from: UniProt accession Q9UEF7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Klotho protein (Tagged-His Tag) images

    • SDS-PAGE - Klotho protein (Tagged-His Tag) (ab124316)
      SDS-PAGE - Klotho protein (Tagged-His Tag) (ab124316)
      12% SDS-PAGE separation of Human Klotho protein (ab124316) on a coomasie stained gel
      Lane 1: reduced and boiled sample (5 µg)
      Lane 2: non-reduced and non-boiled sample (5 µg)

    References for Klotho protein (Tagged-His Tag) (ab124316)

    ab124316 has not yet been referenced specifically in any publications.

    Publishing research using ab124316 ? Please let us know so that we can cite the reference in this datasheet

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    10
    Votes: Highest First

    Sehr geehrte Damen und Herren,

    Ich bin auf der Suche nach rekombinanten bzw natürlichem, aufgereinigten
    humanen alpha Klotho. Bieten Sie noch weitere Domänen/Versionen dieses
    Proteins an (130kDa Form; transmembrane Domän...

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    Vielen Dank für Ihre Anfrage.

    Leider ist das Klotho-Fragment der Aminosäuren 34-549 das einzige Klotho, das wir verfügbar haben. Unter ab84072 bieten wir die ungetagte Form an, unter ab124316 ist das gleiche Fragment mit einem C-t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"