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All lanes : Anti-Kv1.6 antibody (ab21089) at 1 µg/ml
Lane 1 : Mouse Brain Lysate
Lane 2 : Mouse Brain Lysate with
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Lane 2 : Alexa Fluor Goat polyclonal to Rabbit IgG
Predicted band size : 58 kDa
Observed band size : 58 kDa
Additional bands at : 35 kDa (possible cleavage fragment,non-specific binding,degradation product),39 kDa (possible cleavage fragment,non-specific binding,degradation product),45 kDa (possible cleavage fragment,non-specific binding,degradation product).
ab21089 recognises a band corresponding to the predicted MW of Kv1.6 in mouse brain lysate (lane 1). Antibody binding to this band may be blocked using the immunising peptide, ab23431 (lane 2). The antibody also recognises additional proteins. These may be degradation/cleavage products of Kv1.6, or they may represent non-specific binding by the antibody.
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