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L-Carnitine Assay Kit (ab83392)

Q&A (14)

Overview

  • Product nameL-Carnitine Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Range
    1 µM - 200 µM
  • Assay time
    0h 30m
  • Product overview

    Abcam's L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum. The assay transfers an acetyl group from CoA to carnitine and the free CoA formed is further processed with subsequent oxidation of the OxiRed probe to give fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). The normal range for serum L-Carnitine is ~20-100 µM. The detection sensitivity is ~1 µM for the fluorometric assay and ~10 µM for the colorimetric assay.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where beta-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L-Carnitine is often sold as a nutritional supplement. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCarnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where beta oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L Carnitine is often sold as an antioxidant in nutritional supplements. Carnitine exists in two stereoisomers. Only L carnitine is biologically active.
    • Alternative names
        CarnitineL Carnitine

    Applications

    Our Abpromise guarantee covers the use of ab83392 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    L-Carnitine Assay Kit images

    • Functional Studies - L-Carnitine Assay Kit (ab83392)
      Functional Studies - L-Carnitine Assay Kit (ab83392)
      Example of L-Carnitine standard curve obtained using ab83392

    Protocols

    References for L-Carnitine Assay Kit (ab83392)

    ab83392 has not yet been referenced specifically in any publications.

    Publishing research using ab83392 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Please advise regarding item# ab83392 L-carnitines assay kit

    What is the reason for lack in CE certification for this product?

    Is there problem with his suitability to work on human tissue/cells?

    Or due to the fact Abcam manufa...

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    Thank you for contacting us.

    We are not ISO registered company which is why we do not have CE certification. We can however provide certificate of analysis, if requested. I don't think CE certification is required for research products. Our produ...

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    Here are some more questions regarding the kit:

    Is there a way to change the kit's protocol and use it to test total L-Carnitine as well as free L-Carntine?
    What kind of column should be used to deproteinize the serum?
    What are the sp...

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    Thank you for your message and for your patience.

    I have already received information to confirm that this kit has been optimized to measure free L-carnitine and so regrettably we cannot guarantee or advice regarding measurement of total L carn...

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    Is the kit suitable for use with EDTA plasma?



    This kit will be very suitable for use with EDTA plasma samples.

    Dear Tech support

    Regarding kit ab83392.
    How was The normal range for serum L-Carnitine stated in the datasheet determined, can you give relevant references.

    Please advise.

    Thank you for your kind help.

    Reg...

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    Thank you for your enquiry regarding ab83392.

    My colleague Kate has been out of the office this morning. Here are some links which show these levels:

    http://www.kidney.org/professionals/KDOQI/guidelines_ped_ckd/cpr9.htm

    http:...

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    Hello Abcam-Team,
    i have a question concerning your L-Carnitine Assay Kit.
    Does this kit also detect D-Carnitine?
    Is the acetyl transferase specific for the L-form?
    Thanks a lot,
    with kind regards,

    Thank you for your inquiry.

    This kit should be very specific for L-Carnitine since the enzymes used are specific to it. Also the reaction mimics the biological activity of L-Carnitine and only L-Carnitine is active not D-Carnitine.

    It ...

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    I purchased 83392 several months ago. All of the components have been in the refrigerator-but I see I should have frozen some of them??


    Also there is a reference to carnitine assay buffer-is this something I need to buy?


    ...

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    Thank you for contacting Abcam

    Upon arrival the kit has to be stored at -20C. Certain key components in the kit are lyophilized so the kit may be stable. However I cannot guarantee this and you will have to test whether it is still working.
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    Can you please explain how this kit works? What is the specific reaction that takes place? Is the enzyme carnitine acyl transferase?

    Thank you for contacting Abcam regarding ab83392. Unfortunately, the specific details of the reaction mechanism and enzyme names are proprietary information in this case. I can only provide the following: Acetyl CoA + Carnitine ---> Free CoA + Acetylated ...

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    Dear Concerning, A customer would like to use the kit on cattle samples. Is the kit suitable to apply on these sapmles?  Do you have any information from literatures about the issue? If the kit is suitable, Could you please sent a literature informati...

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    Thank you for your enquiry. I am sorry to confirm that as far as we are aware,  this kit has not been specifically used for detection of L-Carnitine Assay  in bovine samples.  We are therefore not able to provide data from this species.  As this is no...

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    Hello,   I’ve been asked to evaluate an ELISA l-carnitine method (non-fluorescent), what items /reagents do you supply?  Do you recommend the indirect or direct method protocol?  I will not need to do a sandwich assay at this point in time.   ...

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    Thank you for contacting Abcam I have looked through our catalogue and I believe that the L-Carnitine Assay Kit (ab83392) that you identified would be the best option to measure carnitine in animal feed. The probe included in the kit can be ...

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    Does this kit detect D-Carnitine as well? If this hasn't been tested, would it be predicted to react?

    Thank you for contacting us. This is a kit that is very specific to L-Carnitine, as the enzymes used in this kit are very specific. We have not tested the cross-reactivity to D-Carnitine, but we would expect it to be minimal. I hope this informa...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"