Overview

  • Product nameL-Carnitine Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Range
    1 µM - 200 µM
  • Assay time
    0h 30m
  • Product overview

    Abcam's L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum. The assay transfers an acetyl group from CoA to carnitine and the free CoA formed is further processed with subsequent oxidation of the OxiRed probe to give fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). The normal range for serum L-Carnitine is ~20-100 µM. The detection sensitivity is ~1 µM for the fluorometric assay and ~10 µM for the colorimetric assay.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where beta-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L-Carnitine is often sold as a nutritional supplement. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCarnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where beta oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L Carnitine is often sold as an antioxidant in nutritional supplements. Carnitine exists in two stereoisomers. Only L carnitine is biologically active.
  • Alternative names
    • Carnitine
    • L Carnitine

Applications

Our Abpromise guarantee covers the use of ab83392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

L-Carnitine Assay Kit images

  • Example of L-Carnitine standard curve obtained using ab83392.

  • Example of L-Carnitine standard curve obtained using ab83392.

Protocols

References for L-Carnitine Assay Kit (ab83392)

ab83392 has not yet been referenced specifically in any publications.

Product Wall

The ab138889 CoA detection kit assay buffer is 100 mM Na phosphate buffer, pH = 6. The RIPA buffer may be an issue with the other two kits, since they are enzyme-based, and that kind of assay is generally incompatible with detergents. Your best approac...

Read More

The instructions in the protocol are correct, and the values are too, 0, 2, 4, 6, 8, and 10 nmol per well. Here is the calculation:

Starting with the 1 mM standard carnitine solution, calculate the moles per microliter.

1 millimol...

Read More

Thank you for contacting us.

We are not ISO registered company which is why we do not have CE certification. We can however provide certificate of analysis, if requested. I don't think CE certification is required for research products. Our pr...

Read More

Thank you for your message and for your patience.

I have already received information to confirm that this kit has been optimized to measure free L-carnitine and so regrettably we cannot guarantee or advice regarding measurement of total L c...

Read More



This kit will be very suitable for use with EDTA plasma samples.

Thank you for your enquiry regarding ab83392.

My colleague Kate has been out of the office this morning. Here are some links which show these levels:

http://www.kidney.org/professionals/KDOQI/guidelines_ped_ckd/cpr9.htm

ht...

Read More

Thank you for your inquiry.

This kit should be very specific for L-Carnitine since the enzymes used are specific to it. Also the reaction mimics the biological activity of L-Carnitine and only L-Carnitine is active not D-Carnitine.

...

Read More

Thank you for contacting Abcam

Upon arrival the kit has to be stored at -20C. Certain key components in the kit are lyophilized so the kit may be stable. However I cannot guarantee this and you will have to test whether it is still working. <...

Read More

Thank you for contacting Abcam regarding ab83392. Unfortunately, the specific details of the reaction mechanism and enzyme names are proprietary information in this case. I can only provide the following: Acetyl CoA + Carnitine ---> Free CoA + Acetylat...

Read More

Thank you for your enquiry. I am sorry to confirm that as far as we are aware,  this kit has not been specifically used for detection of L-Carnitine Assay  in bovine samples.  We are therefore not able to provide data from this species.  As this is...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"