Overview

  • Product nameAnti-L Glutamate antibody
    See all L Glutamate primary antibodies
  • Description
    Rabbit polyclonal to L Glutamate
  • SpecificityThe antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000) was detected against glutamate in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, D/L-aspartate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) was detected against D-glutamate. Significant cross-reactivity (1:8) was detected against free NAAG in competition assays (NAAG is not retained in aldehyde-fixed tissue).
  • Tested applicationsIHC-FoFr, IHC-Fr, ICC, Immunomicroscopy, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    L-glutamate conjugated to glutaraldehyde

  • General notesThe product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.

Properties

Applications

Our Abpromise guarantee covers the use of ab9440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur.
IHC-Fr Use at an assay dependent concentration.
ICC 1/100. See protocol. OPTIMAL FIXATION: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI protocol (The antisera targets the glutaraldehyde conjugate of the hapten). All procedures may be carried out at room temperature. MINIMAL GLUTARALDEHYDE: 0.00% using minimum glutaraldehyde Enhanced HPI (EHPI) protocol with 4% formaldehyde.
Immunomicroscopy Use at an assay dependent concentration.
ICC/IF 1/100.
IHC-P Use at an assay dependent concentration.

Target

  • RelevanceL Glutamate is a non-essential amino acid naturally occurring in the L-form. It is the most common excitatory neurotransmitter in the Central Nervous System.
  • Alternative names
    • Glutamate antibody
    • L glutamate antibody

Anti-L Glutamate antibody images

  • ab9440 staining L Gluramate in Mouse retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol, permeabilized with 0.025% TBS-Triton X-100 and blocked with 5% serum for 1 hour. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 18 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-L Glutamate antibody (ab9440)

This product has been referenced in:
  • Wu Z  et al. Tonic inhibition in dentate gyrus impairs long-term potentiation and memory in an Alzhiemer's disease model. Nat Commun 5:4159 (2014). Read more (PubMed: 24923909) »
  • Li D  et al. Optogenetic activation of LiGluR-expressing astrocytes evokes anion channel-mediated glutamate release. J Physiol 590:855-73 (2012). Read more (PubMed: 22219341) »

See all 7 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Cell (retina)
Specification retina
Permeabilization Yes - 0.3% TritonX-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 14 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: amb°C
Sample Mouse Tissue sections (retina)
Specification retina
Permeabilization Yes - TBS-Tritòn X-100 0,025 % 15 min
Fixative Methanol
Username

Dr. Patricia Bogdanov

Verified customer

Submitted Jul 15 2013

Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Mar 05 2013

Thank you for contacting us.
Here are the references we spoke about on the phone for this product: http://www.abcam.com/L-Glutamate-antibody-ab9440-references.html
Some other background references might be helpful as well to see what other la...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.3% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Jan 24 2013

Thank you for contacting us and for your interest in our products. I can confirm that ab9440L Glutamate antibody is tested and covered by our guarantee for IHC-FoFr, IHC-Fr, ICC, Immunomicroscopy andIHC-P. I am sorry it has not been tested in ELISA or ...

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Thanks for your call today and for your patience while I have looked into your question. I found that the antibody ab8893 does not react with free L-glutamate so I don't believe it will be suitable for the ELISA assay that you are planning. We d...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (spinal cord)
Specification spinal cord
Fixative Paraformaldehyde
Antigen retrieval step None
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. Travis Lyttle

Verified customer

Submitted Jan 08 2007

I have received some further information regarding this antibody from the priginator. Ab9440 does not bind any anti-mouse product that has been tested. The only possible explanation is that the antibody was denatured and that it exposed a non-specific ...

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Thank you for your enquiry. Unfortunately, we are not aware of any publications that feature the use of this antibody. When we are aware of such references, we cite them on the online datasheets. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"