• Product name
    L-Lactate Assay Kit (Colorimetric)
    See all L-Lactate kits
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
  • Assay type
  • Sensitivity
    0.02 mM
  • Range
    0.02 mM - 10 mM
  • Assay time
    0h 40m
  • Product overview

    L-Lactate Assay Kit (Colorimetric) (ab65331) is an assay where lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (λmax = 450 nm). The kit detects L(+)-Lactate in biological samples such as serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.

    Visit our FAQs page for tips and troubleshooting.

    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • Notes

    L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate.

  • Tested applications
    Suitable for: Functional Studiesmore details


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    L(+)-Lactate Standard (100 nmol/µl) Yellow 1 x 100µl
    Lactate Assay Buffer WM 1 x 25ml
    Lactate Enzyme Mix (lyophilized) Green 1 vial
    Lactate Substrate Mix Red 1 vial
  • Research areas
  • Relevance
    Lactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).

Associated products


Our Abpromise guarantee covers the use of ab65331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.


  • Plasma lactate concentrations were determined using L-Lactate assay kit (ab65331) in Ark2C+/+ and Ark2C−/− (Arkadia-like gene) mice.

  • Linearity of dilution: concentration of L-Lactate in differently diluted (X-axis) biological samples, demonstrating a linearity of 89%-111% (concentrations corrected for by factor of dilution; duplicates; +/- SD).

  • Relative signal (RFU) in unfiltered human plasma (dilution 1:8), comparing L-lactate signals with background reading (no enzyme) after 10 minutes of incubation (duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Lactate Standard Curve. The assay is performed following the kit (ab65331) protocol.



This product has been referenced in:
  • Sinha PB  et al. MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation. J Ovarian Res 10:37 (2017). Read more (PubMed: 28629378) »
  • Robinson MD  et al. Water T2as an early, global and practical biomarker for metabolic syndrome: an observational cross-sectional study. J Transl Med 15:258 (2017). Read more (PubMed: 29258604) »

See all 28 Publications for this product

Customer reviews and Q&As

Merci pour cette information. Je suis très désolée que ces kits n'ont pas permis de faire les dosages attendus. Je vais volontiers organiser une note de crédit ou un remboursement pour vous. Alternativement je peux aussi org...

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For cell samples: Start with ˜2x106 cells, suspend the cell pellet 500 μl (or ˜4 volumes) of the Assay Buffer on ice, homogenize using a Dounce homogenizer (10-50 passes) on ice, until efficient lysis is confirmed, by viewing the cells under the mic...

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I can confirm that this kit (ab6533:L-Lactate Assay Kit) uses 450 nm absorbance reading and hence the color should not affect the measurements.

For those kits that measure absorbance at 570 nm, the red color could potentially interfe...

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Yes, theoretically it can be done by monitoring the changes in NADH absorption at 340 nm.

However, measuring at this OD requires expensive quartz microplates/cuvettes.
Enzymatic assays like ab65331 are a better way to get more accurate ...

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The standard is 98% pure sodium-L-lactate (chemical, not animal derived).

Thank you for your enquiry.

For this kit (ab65331) 10-100mg tissue can be used depending upon the lactate in the tissue. This will require some optimization depending on the sample. We recommend using different dilutions of the homogenate so ...

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L-lactate assay kit

Good Good 4/5 (Ease of Use)
The assay kit is a fast and easy way to quantify lactate concentrations. It works well for whole cell lysates (WCE). HCT116 cells were cultivated for 48 and 72 hrs in DMEM and sonified in assay buffer. The lactate concentration is displayed in the enclosed figure, dependent on the amount of WCE.
The kit only got 4 stars because it does not work in DMEM thus the supernatant of the cells could not be analysed. 10 µl DMEM in combination with assay buffer and the reaction mix gave absorbance values over 1.

Mr. Christian Marx

Verified customer

Submitted Jul 23 2013

Thank you for contacting us.

Fetal Bovin Serum contains LDH and will create high background levels. Using a low percent FBS (0.25-1%) or serum free media will reduce this background.

I hope this information is helpful to you. Plea...

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Yes, you can use the syringe. Also use vortexing and sonication alongside it for efficient homogenization. Check the homogenization efficiency under the microscope.

Yes, our ab65330 L-Lactate Assay Kit (Fluorometric) can be used for colorimetric detection. The difference this kit has with the ab65331 L-Lactate Assay Kit (Colorimetric) is indeed mainly the probe, but the detection limits are also different for...

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1-10 of 62 Abreviews or Q&A


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