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Overview

  • Product nameL-Lactate Assay Kit (Colorimetric)See all Lactate kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • Assay typeQuantitative
  • Sensitivity
    0.02 mM
  • Range
    0.02 mM - 10 mM
  • Assay time
    0h 40m
  • Product overview

    In Abcam's L-Lactate Assay Kit (Colorimetric), lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (?max = 450 nm). The kit detects L(+)-Lactate in biological samples such as serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate.

  • Tested applicationsFunctional Studies more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    L(+)-Lactate Standard (100 nmol/µl) Yellow 1 x 100µl
    Lactate Assay Buffer WM 1 x 25ml
    Lactate Enzyme Mix (lyophilized) Green 1 vial
    Lactate Substrate Mix Red 1 vial
  • Research Areas
  • RelevanceLactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).

    • Applications

    Our Abpromise guarantee covers the use of ab65331 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    L-Lactate Assay Kit (Colorimetric) images

    • Functional Studies - Lactate Assay Kit II (ab65331)
      Functional Studies - Lactate Assay Kit II (ab65331)
      Lactate Standard Curve. The assay is performed following the kit (ab65331) protocol.

    Protocols

    References for L-Lactate Assay Kit (Colorimetric) (ab65331)

    This product has been referenced in:
    • Yang J  et al. Human CHCHD4 mitochondrial proteins regulate cellular oxygen consumption rate and metabolism and provide a critical role in hypoxia signaling and tumor progression. J Clin Invest 122:600-11 (2012). Read more (PubMed: 22214851) »
    • Demaria M  et al. STAT3 can serve as a hit in the process of malignant transformation of primary cells. Cell Death Differ : (2012). Read more (PubMed: 22402588) »

    See all 7 Publications for this product

    Publishing research using ab65331 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab65331 Submit a Question for ab65331

    Displaying 1 - 10 of 53 results for Abreviews and Q&A

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    Votes: Highest First

    Hi,

    I was looking for D-lactate assay kit and came to L-Lactate with Abcam. Would you please kindly advice me on the difference between D & L-Lactate and their measurement in clinical application. I know that D is at lower concentration vs ...

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    Thank you for contacting us. Yes, D-lactate is produced in very low levels in mammals unless there are certain bacterial infections or trauma. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in bloo...

    Read More

    1. What is the principle of the assay?
    2. Is there any data for levels of lactate in normal mouse plasma?

    The LDH oxidation of lactate to pyruvate produces NADH which in turn reduces the WST (water soluble tetrazolium) to formazan to create the colored end product.

    The only data I am aware of for mouse serum or plasma is in the reference listed on ...

    Read More

    Thank you. We tried this assay and basically the results were confounded (and obscured) by the
    interference of bubbles with the spec readings. Bubbles were produced when the buffer was pipetted
    into each well. This seems to be due to the fact tha...

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    Thank you for your reply.
    When pipetting, you can touch the pipet tip to the side of the wall to decrease bubble formation. Alternatively, you can use the end of a clean paper clip to pop the bubbles in the wells before reading. This is a common proc...

    Read More


    I'm interested in the ab65331 product to detect lactate. Specifically, I would like to detect lactate production in flow sorted mouse cells. The sort yield is typically around 50K cells, and I'm wondering whether the total lactate produced in these c...

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    50k cells should fall within the limit of detection. However I would start by doing a 30k and a 50K sample preparation to optimize.
    Lysing the samples well with numerous strokes using a dounce homogenizer should be able to release the lactate.

    Which salt of lactic acid is the standard?

    The standard is sodium lactate.

    What is the limit of detection for this assay? We need to measure nmol/ml in mouse urine.

    L-Lactate Assay Kit (Colorimetric) (ab65331)

    Thank you for your inquiry.
    The range of detection for this kit is 0.02 mM - 10 mM.
    You can see the nmol lactate in the standard curve diagram on the datasheet. http://www.abcam.com/l-lactate-assay-kit-colorimetric-ab65331.html
    The standard...

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    The customer is interested in the replacement kit. Please add the replacement ab65331 to Zotal's current week order.

    Please confirm.

    Thanks in advance for your assistance and reply.

    Kind Regards,

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this kit did not perform as stated on the datasheet and for the inconvenience this has ca...

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    Dear Kate,

    Please see below the answers from the customer:

    1. Reviewing the data, the samples as well as the standards are showing similar OD values. Therefore, it may be possible that the reconstitution or storage of at least one of...

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    Thank you for your reply.

    I appreciate the time the customer has spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provid...

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    Please read below customer's answers and please advise:

    - How was the kit stored?
    -20

    - Are the blank readings as expected?
    Yes but there was no change in OD measurements when the standard was added.

    What do you ...

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    Thank you for taking the time to complete this information. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    I have reviewed this case with t...

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    Thank you for your prompt reply. I think that some misunderstanding occurred: there is a problem with the standards (see the attached file, yellow cells indicate 3 repeats of the standards reading). There is no logical difference in the readings between d...

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    Thank you for your reply.

    I understand that it is the standard that is causing the problems in this case. Collecting general information is helpful for our quality monitoring and general investigation of a case.

    On this occasion, I w...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"