L-Lactate Assay Kit (Fluorometric) (ab65330)
- Product nameL-Lactate Assay Kit (Fluorometric)See all Lactate kits ...
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeQuantitative
- Sensitivity> 0.001 mM
- Range0.001 mM - 10 mM
- Assay time0h 40m
- Species reactivityReacts with: all Mammals
- Product overview
In Abcam's L-Lactate Assay Kit (Fluorometric), lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm). The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples.
Visit our FAQs page for tips and troubleshooting.
L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests L(+)-Lactate Standard (100 nmol/µl) Yellow 1 x 100µl Lactate Assay Buffer WM 1 x 25ml Lactate Enzyme Mix (lyophilized) Green 1 vial Lactate Probe (in DMSO, anhydrous) Red 1 x 200µl
- Research Areas
Our Abpromise guarantee covers the use of ab65330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for L-Lactate Assay Kit (Fluorometric) (ab65330)
This product has been referenced in:
- Agathocleous M et al. Metabolic differentiation in the embryonic retina. Nat Cell Biol 14:859-64 (2012). Xenopus tropicalis . Read more (PubMed: 22750943) »
- Suzuki A et al. Astrocyte-neuron lactate transport is required for long-term memory formation. Cell 144:810-23 (2011). Read more (PubMed: 21376239) »