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Overview

  • Product nameL-Lactate Assay Kit (Fluorometric)See all Lactate kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Sensitivity
    > 0.001 mM
  • Range
    0.001 mM - 10 mM
  • Assay time
    0h 40m
  • Species reactivity
    Reacts with: all Mammals
  • Product overview

    In Abcam's L-Lactate Assay Kit (Fluorometric), lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm). The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate.

  • Tested applicationsFunctional Studies more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    L(+)-Lactate Standard (100 nmol/µl) Yellow 1 x 100µl
    Lactate Assay Buffer WM 1 x 25ml
    Lactate Enzyme Mix (lyophilized) Green 1 vial
    Lactate Probe (in DMSO, anhydrous) Red 1 x 200µl
  • Research Areas
  • RelevanceLactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).

    • Applications

    Our Abpromise guarantee covers the use of ab65330 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    L-Lactate Assay Kit (Fluorometric) images

    • Functional Studies - L-Lactate Assay Kit (Fluorometric) (ab65330)
      Functional Studies - L-Lactate Assay Kit (Fluorometric) (ab65330)
      Standard calibration Curve of Lactate.

    Protocols

    References for L-Lactate Assay Kit (Fluorometric) (ab65330)

    This product has been referenced in:

    See all 4 Publications for this product

    Publishing research using ab65330 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab65330 Submit a Question for ab65330

    Displaying 1 - 10 of 19 results for Abreviews and Q&A

    10
    Votes: Highest First

    I’ve been using your L-Lactate assay kit (ab65330), Glucose assay kit (ab65333) and Free Glycerol detection kit (ab65337) to collect data. I had no problem with measurement except 1 series of samples. I would like to modify the condition to see whet...

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    I cannot give the exact compositions of the enzyme mixes in these kits, but the probes are just pure probes in a solvent. They do not have any other components. The enzyme mixes are obviously a combination of multiple enzymes. It does not have any floresc...

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    If so, how long should we have incubated the samples before reading the plate? Should we rerun our experiment in order to obtain data that we can accurately

    Thank you for contacting us. It should have mentioned in the protocol the incubation time is 30 minutes. We will be sure to correct the protocol.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any...

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    Thanks for your answer.
    My concern was that using the assay buffer to homogenize the samples might generate a lot of bubbles (if the buffer has any kind of detergent).
    Can I extract lactate from samples using ethanol 80% and remove the proteins w...

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    Thanks for your response.
    I strongly recommend sticking to the kit protocol. Adding solutions (such as ethanol and perchloric acid) other than those provided in the kit will compromise the efficiency of lactate detection. The centrifugation step can b...

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    Product code: 65330
    Lot number: XXXXXX
    º
    Inquiry: Hi, We just bought the L-Lactate assay kit and I cannot find in the assay protocol how should I homogenize my tissue samples (hypothalamus). I will apreciate information about this issue i...

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    Thank you for contacting Abcam.
    Since the lactate dehydrogenase (LDH) in tissue lysates degrades lactate, be sure to keep your samples at -80ºC. Below is our recommended homogenization protocol:
    1. Homogenize the tissue samples (should contain at ...

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    Product code: 65330

    Inquiry: I would like to try the lactate assay kit ≠ab65330 for determination of intracellular lactate content. I have to work in 96well plate and I want to know whether using a standard lysis buffer (say with 0.5% Triton...

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    Thank you for your enquiry.

    We would recommend to use the assay buffer provided with ab65330 L-Lactate Assay Kit (Fluorometric) for cells. This buffer contains some proprietary ingredients which are crucial for the working efficiency of this as...

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    How to prepare cell culture extracts for use in this assay?

    Thank you for contacting Abcam earlier today.

    To prepare an intracellular lysate to use in the assay, we recommend using at least 2 x 10^6 cells. Then homogenize the cells in the assay buffer that is provided and centrifuge it gently to get rid o...

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    Hola buenas tardes,
    Me podrias Informar a que temperatura debe conservarse este KIT y la fecha de Vencimiento¿
    L-Lactate Assay Kit (Fluorometric) (ab65330), http://www.abcam.com/L-Lactate-Assay-Kit-Fluorometric-ab65330-support.html
    D...

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    Gracias por contactarnos.

    El kit de detección de Lactato debe mantenerse a -20C. Almacenado a esta temperatura y evitando ciclos de congelación y descongelación, el kit dura hasta la fecha indicada en la caja del kit, que su...

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    I am working in a research project and I need to measure the levels of D-lactate and L-lactate in MRSB culture supernatants, are the kits sale by your company compatible with this type of media . Is there any interference due to the media color?

    Thank you for contacting us.

    I am not familiar with the contents of MRSB media, but in general cell culture supernatants will be compatible with these kits. If the media contains serum, we recommend deproteinizing the samples using our 10 kDa cu...

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    To whom it may concern,

    I am interested in your L-Lactate Assay kit (Fluorometric) cat# ab65330

    I will be using this as a cell based assay and was wondering if you could recommend a positive control cell line and or any specific reag...

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    Thank you for your inquiry.

    I can suggest to use muscle or glial cells as positive control. I do not know any specific cell culture cell line that could be used but a literature search might help to find some more information on that.

    ...

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    I am currently trying to use your Pyruvate and Lactate assay kits (ab65330 and ab65342) to measure intracellular levels of these metabolites. I have two questions based on logistics of getting usable results from the assay.

    The first several ti...

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    Thank you for contacting Abcam.

    Use at least 2 x 10^6 cells. Homogenize the cells in the assay buffer and centrifuge it gently to get rid of the foam/air bubbles. We suggest using several doses of your sample to ensure the readings are within st...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"