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ab24345 has been referenced in 11 publications.
Publishing research using ab24345? Please let us know so that we can cite the reference in this datasheet
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ab24345 (1/500) immunostaining L1CAM in 4% PFA-fixed rat cerebellum tissue. ML = molecular layer and FT = fiber tract of the cerebellum.
Martin Grumet, Rutgers University, United States
Anti-L1CAM antibody [2C2] - Neuronal Marker (ab24345) at 1/1000 dilution + 30ug CNS protein
Performed under reducing conditions.
Observed band size : 200 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60-80 kDa (possible cleavage fragment).
ab24345 recognizes one or two polypeptides of L1 or Ng-CAM corresponding to the full length protein (~200kDa) as well as 60-80 kDa C-terminal cleavage products (as shown in the figure).
Martin Grumet, Rutgers University, United States
Overlay histogram showing PC12 cells stained with ab24345 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24345, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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