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Anti-L1CAM antibody [2C2] - Neuronal Marker (ab24345)

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Overview

Product name

Anti-L1CAM antibody [2C2] - Neuronal Marker
See all L1CAM products (12) ...

Description

Mouse monoclonal [2C2] to L1CAM - Neuronal Marker

Specificity

ab24345 L1[2C2] antibody recognizes one or two polypeptides of L1 or Ng-CAM corresponding to the full length protein (~200 kD) and the 60-80 kD C-terminal cleavage products (as shown). ab24345 will recognize protein after denaturation in the presence of reducing agents and can detect protein in lysates (10-100 micrograms of protein) of the nervous system (such as rat cerebellum - see figure).

Tested applications

ICC/IF, IHC-FoFr, Flow Cyt, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

chicken NgCAM protein (ab24345 detects the C-terminus portion of the protein that is conserved with mammalian L1)

Positive control

rat cerebellum, nervous system tissue

General notes

L1CAM can be detected between 200-220 kD. In brain samples it is typically seen at ~ 200 kD. When the protein is overexpressed in vitro it is often detected as a doublet with bands at 200 and 220 kD. The unglycosylated, unprocessed L1CAM is ~ 140-150 kDa. The protein has 21 putative N-glycosylation sites on the extracellular portion of the protein which, when they are all glycosylated, results in a detected MW of 200-220 kD depending upon how many residues are actually glycosylated. L1CAM can be cleaved by the metalloprotease ADAM10 resulting in fragments of 180 kD and 40 kD. L1CAM can also be cleaved by plasmin resulting in fragments of 140 kD and 80 kD. In theory, therefore, one could detect bands at ~220, 200, 180, 140, 80 and 40 kD.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Liquid ascites fluid that was precipitated by ammonium sulfate and resuspended and dialyzed in PBS.

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

2C2

Isotype

IgG1

Research areas

Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Liver

  • Immunohistochemistry - L1CAM antibody [2C2] - Neuronal Marker (ab24345)Immunohistochemistry - L1CAM antibody [2C2] - Neuronal Marker (ab24345) image (enlarge)

  • Western blot - L1CAM antibody [2C2] - Neuronal Marker (ab24345)Western blot - L1CAM antibody [2C2] - Neuronal Marker (ab24345) image (enlarge)

  • Flow Cytometry - L1CAM antibody [2C2] - Neuronal Marker (ab24345)Flow Cytometry - L1CAM antibody [2C2] - Neuronal Marker (ab24345) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab24345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.

Involvement in disease

Defects in L1CAM are the cause of hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS) [MIM:307000]. Hydrocephalus is a condition in which abnormal accumulation of cerebrospinal fluid in the brain causes increased intracranial pressure inside the skull. This is usually due to blockage of cerebrospinal fluid outflow in the brain ventricles or in the subarachnoid space at the base of the brain. In children is typically characterized by enlargement of the head, prominence of the forehead, brain atrophy, mental deterioration, and convulsions. In adults the syndrome includes incontinence, imbalance, and dementia. HSAS is characterized by mental retardation and enlarged brain ventricles.
Defects in L1CAM are the cause of mental retardation-aphasia-shuffling gait-adducted thumbs syndrome (MASA) [MIM:303350]; also known as corpus callosum hypoplasia, psychomotor retardation, adducted thumbs, spastic paraparesis, and hydrocephalus or CRASH syndrome. MASA is an X-linked recessive syndrome with a highly variable clinical spectrum. Main clinical features include spasticity and hyperreflexia of lower limbs, shuffling gait, mental retardation, aphasia and adducted thumbs. The features of spasticity have been referred to as complicated spastic paraplegia type 1 (SPG1). Some patients manifest corpus callosum hypoplasia and hydrocephalus. Inter- and intrafamilial variability is very wide, such that patients with hydrocephalus, MASA, SPG1, and agenesis of corpus callosum can be present within the same family.
Defects in L1CAM are the cause of spastic paraplegia X-linked type 1 (SPG1) [MIM:303350]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Note=Defects in L1CAM may contribute to Hirschsprung disease by modifying the effects of Hirschsprung disease-associated genes to cause intestinal aganglionosis.
Defects in L1CAM are a cause of partial agenesis of the corpus callosum (ACCPX) [MIM:304100]. A syndrome characterized by partial corpus callosum agenesis, hypoplasia of inferior vermis and cerebellum, mental retardation, seizures and spasticity. Other features include microcephaly, unusual facies, and Hirschsprung disease in some patients.

Sequence similarities

Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Cellular localization

Cell membrane.

Target information above from: UniProt accessionP32004 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • antigen identified by monoclonal antibody R1 antibody
  • CAML1 antibody
  • CD 171 antibody
  • CD171 antibody
  • CD171 antigen antibody
  • HGNC:7086 antibody
  • HSAS antibody
  • HSAS1 antibody
  • Hyd antibody
  • L1 antibody
  • L1 cell adhesion molecule antibody
  • L1-NCAM antibody
  • L1cam antibody
  • L1CAM_HUMAN antibody
  • MASA antibody
  • MIC 5 antibody
  • MIC5 antibody
  • N CAM L1 antibody
  • N CAML1 antibody
  • N-CAM-L1 antibody
  • NCAM-L1 antibody
  • NCAML1 antibody
  • Nerve-growth factor-inducible large external glycoprotein antibody
  • Neural cell adhesion molecule L1 antibody
  • NEURAL CELL ADHESION MOLECULE L1 PRECURSOR antibody
  • NILE antibody
  • OTTHUMP00000025992 antibody
  • S10 antibody
  • SPG 1 antibody
  • SPG1 antibody
see all

Database links

see all

Anti-L1CAM antibody [2C2] - Neuronal Marker images:

  Immunohistochemistry - L1CAM antibody [2C2] - Neuronal Marker (ab24345)

Immunohistochemistry - L1CAM antibody [2C2] - Neuronal Marker (ab24345)

ab24345 (1/500) immunostaining L1CAM  in 4% PFA-fixed rat cerebellum tissue. ML = molecular layer and FT = fiber tract of the cerebellum.

Martin Grumet, Rutgers University, United States

  Western blot - L1CAM antibody [2C2] - Neuronal Marker (ab24345)

Western blot - L1CAM antibody [2C2] - Neuronal Marker (ab24345)

Anti-L1CAM antibody [2C2] - Neuronal Marker (ab24345) at 1/1000 dilution + 30ug CNS protein

Performed under reducing conditions.

Observed band size : 200 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60-80 kDa (possible cleavage fragment).

ab24345 recognizes one or two polypeptides of L1 or Ng-CAM corresponding to the full length protein (~200kDa) as well as 60-80 kDa  C-terminal cleavage products (as shown in the figure).

Martin Grumet, Rutgers University, United States

  Flow Cytometry - L1CAM antibody [2C2] - Neuronal Marker (ab24345)

Flow Cytometry - L1CAM antibody [2C2] - Neuronal Marker (ab24345)

Overlay histogram showing PC12 cells stained with ab24345 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24345, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with methanol (5 min) used under the same conditions.

Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-L1CAM antibody [2C2] - Neuronal Marker (ab24345)

This product has been referenced in:

  • Hu XLet al. Conditional Deletion of NRSF in Forebrain Neurons Accelerates Epileptogenesis in the Kindling Model. Cereb Cortex : (2011).Read more (PubMed: 21339379) »
  • Guan H & Maness PF Perisomatic GABAergic innervation in prefrontal cortex is regulated by ankyrin interaction with the L1 cell adhesion molecule. Cereb Cortex 20:2684-93 (2010). Mouse.Read more (PubMed: 20156840) »

See all 11 publications for this product

Publishing research using ab24345? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"