L1CAM antibody [2C2] - Neuronal Marker (ab24345)

Product Name 

L1CAM antibody [2C2] - Neuronal Marker

Product type 

Primary antibodies

Description 

Mouse monoclonal [2C2] to L1CAM - Neuronal Marker

Immunogen 

chicken NgCAM protein (ab24345 detects the C-terminus portion of the protein that is conserved with mammalian L1)

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab24345 L1[2C2] antibody recognizes one or two polypeptides of L1 or Ng-CAM corresponding to the full length protein (~200 kD) and the 60-80 kD C-terminal cleavage products (as shown). ab24345 will recognize protein after denaturation in the presence of reducing agents and can detect protein in lysates (10-100 micrograms of protein) of the nervous system (such as rat cerebellum - see figure).

Tested applications 
(see key)

ICC/IF, IHC-FoFr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/300 - 1/1000.
IHC-FoFr: 1/300 - 1/1000.
WB: 1/1000. Detects a band of approximately 200 kDa. Cleavage products observed at 60-80 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

rat cerebellum, nervous system tissue

Cellular localization 

Cell membrane; Single-pass type I membrane protein.

Research areas 

Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Liver

Relevance 

The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

2C2

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Liquid ascites fluid that was precipitated by ammonium sulfate and resuspended and dialyzed in PBS.

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

L1CAM can be detected between 200-220 kD. In brain samples it is typically seen at ~ 200 kD. When the protein is overexpressed in vitro it is often detected as a doublet with bands at 200 and 220 kD. The unglycosylated, unprocessed L1CAM is ~ 140-150 kDa. The protein has 21 putative N-glycosylation sites on the extracellular portion of the protein which, when they are all glycosylated, results in a detected MW of 200-220 kD depending upon how many residues are actually glycosylated. L1CAM can be cleaved by the metalloprotease ADAM10 resulting in fragments of 180 kD and 40 kD. L1CAM can also be cleaved by plasmin resulting in fragments of 140 kD and 80 kD. In theory, therefore, one could detect bands at ~220, 200, 180, 140, 80 and 40 kD.

See below for L1CAM antibody [2C2] - Neuronal Marker images, references, products related to ab24345 and other tools.

L1CAM antibody [2C2] - Neuronal Marker images:

References for L1CAM antibody [2C2] - Neuronal Marker (ab24345)

  This product has been used in: (two most recent references)

Buhusi M et al. L1 interaction with ankyrin regulates mediolateral topography in the retinocollicular projection. J Neurosci 28:177-88 (2008). PubMed: 18171935

Zecchini S et al. The differential role of L1 in ovarian carcinoma and normal ovarian surface epithelium. Cancer Res 68:1110-8 (2008). PubMed: 18281486

See all 8 publication references for this product.


If you publish research using ab24345 please let us know so that we can cite the reference on this datasheet.

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