L1CAM antibody [UJ127.11] (ab20148)

Product Name 

L1CAM antibody [UJ127.11]

Product type 

Primary antibodies

Description 

Mouse monoclonal [UJ127.11] to L1CAM

Immunogen 

Tissue preparation: Homogenous suspension of 16 week human foetal brain

Reacts with 
(species key)

Hu

Specificity 

UJ127.11 may be useful in the diagnosis of embryonic tumours (e.g. neuroblastoma).

Tested applications 
(see key)

IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 200-220 kDa. It may also detect smaller cleavage fragments (please see Notes below).
IHC-P: Use at an assay dependent dilution (from PubMed: 17211730).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell membrane; Single-pass type I membrane protein.

Research areas 

Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Liver

Relevance 

The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

UJ127.11

Myeloma 

P3x63-Ag8.653

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Concentration 

Batch dependent within range: 1.20 - 1.20 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

L1CAM can be detected between 200-220 kD. In brain samples it is typically seen at ~ 200 kD. When the protein is overexpressed in vitro it is often detected as a doublet with bands at 200 and 220 kD. The unglycosylated, unprocessed L1CAM is ~ 140-150 kDa. The protein has 21 putative N-glycosylation sites on the extracellular portion of the protein which, when they are all glycosylated, results in a detected MW of 200-220 kD depending upon how many residues are actually glycosylated. L1CAM can be cleaved by the metalloprotease ADAM10 resulting in fragments of 180 kD and 40 kD. L1CAM can also be cleaved by plasmin resulting in fragments of 140 kD and 80 kD. In theory, therefore, one could detect bands at ~220, 200, 180, 140, 80 and 40 kD.

See below for L1CAM antibody [UJ127.11] images, references, products related to ab20148 and other tools.

L1CAM antibody [UJ127.11] images:

References for L1CAM antibody [UJ127.11] (ab20148)

  This product has been used in: (two most recent references)

Boo YJ et al. L1 expression as a marker for poor prognosis, tumor progression, and short survival in patients with colorectal cancer. Ann Surg Oncol 14:1703-11 (2007). PubMed: 17211730

Patel K et al. Monoclonal antibody UJ127.11 recognizes the human homologue of mouse L1 cell adhesion molecule. Biochem Soc Trans 18:274 (1990). PubMed: 2379713

See all 3 publication references for this product.


If you publish research using ab20148 please let us know so that we can cite the reference on this datasheet.

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