Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Liver
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Is the epitope for LICAM antibody UJ127 intracellular or extracellular? I am looking for an antibody to an extracellular L1 epitope, thanks, |
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ANSWER: |
The cellular localization of L1CAM is cell membrane. The epitope has not yet been determined. |
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I am looking for an L1 antibody for western blots made against rat L1. I can not find it on your seb site. Can you help?
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ANSWER: |
Thank you for your enquiry. I did a search on our website and the only L1CAM antibody that we currently have is ab3200. To our knowledge, cross reactivity with rat has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. To see if another company may have what you are looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249). If you have any more questions, please contact us again. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab3200 at a dilution of 1/1000, staining L1CAM (green; Alexa 488 secondary at 1/2000) on 30
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
Anti-L1CAM antibody [UJ127] (ab3200) at 1 µg/ml +
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 230 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
L1CAM contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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