![Immunocytochemistry/ Immunofluorescence - L1CAM antibody [UJ127] (ab3200) image](#ab3200_1.jpg)
![Western blot - L1CAM antibody [UJ127] (ab3200) image](#L1CAM-Primary-antibodies-ab3200-2.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Liver
Overview
Product name
Anti-L1CAM antibody [UJ127]
See all L1CAM products (12) ...
Description
Mouse monoclonal [UJ127] to L1CAM
Specificity
ab3200 is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin. It does not bind to pediatric or adult brain.
Tested applications
IF, IHC-P, IP, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Homogenous suspension of 16 week human fetal brain.
Positive control
IMR-5 cells.
General notes
L1CAM can be detected between 200-220 kD. In brain samples it is typically seen at ~ 200 kD. When the protein is overexpressed in vitro it is often detected as a doublet with bands at 200 and 220 kD. The unglycosylated, unprocessed L1CAM is ~ 140-150 kDa. The protein has 21 putative N-glycosylation sites on the extracellular portion of the protein which, when they are all glycosylated, results in a detected MW of 200-220 kD depending upon how many residues are actually glycosylated. L1CAM can be cleaved by the metalloprotease ADAM10 resulting in fragments of 180 kD and 40 kD. L1CAM can also be cleaved by plasmin resulting in fragments of 140 kD and 80 kD. In theory, therefore, one could detect bands at ~220, 200, 180, 140, 80 and 40 kD.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
UJ127
Isotype
IgG1
Light chain type
kappa
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Liver
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF
IF: 1/500 - 1/1000.IF: 1/500 - 1/1000.
IHC-P: Use a concentration of 1 - 2 µg/ml.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at 2 µg/mg of lysate.
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 200-220 kDa.( It may also detect smaller cleavage fragments (please see Notes below).)
Target
Function
Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.
Involvement in disease
Defects in L1CAM are the cause of hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS) [MIM:307000]. Hydrocephalus is a condition in which abnormal accumulation of cerebrospinal fluid in the brain causes increased intracranial pressure inside the skull. This is usually due to blockage of cerebrospinal fluid outflow in the brain ventricles or in the subarachnoid space at the base of the brain. In children is typically characterized by enlargement of the head, prominence of the forehead, brain atrophy, mental deterioration, and convulsions. In adults the syndrome includes incontinence, imbalance, and dementia. HSAS is characterized by mental retardation and enlarged brain ventricles.
Defects in L1CAM are the cause of mental retardation-aphasia-shuffling gait-adducted thumbs syndrome (MASA) [MIM:303350]; also known as corpus callosum hypoplasia, psychomotor retardation, adducted thumbs, spastic paraparesis, and hydrocephalus or CRASH syndrome. MASA is an X-linked recessive syndrome with a highly variable clinical spectrum. Main clinical features include spasticity and hyperreflexia of lower limbs, shuffling gait, mental retardation, aphasia and adducted thumbs. The features of spasticity have been referred to as complicated spastic paraplegia type 1 (SPG1). Some patients manifest corpus callosum hypoplasia and hydrocephalus. Inter- and intrafamilial variability is very wide, such that patients with hydrocephalus, MASA, SPG1, and agenesis of corpus callosum can be present within the same family.
Defects in L1CAM are the cause of spastic paraplegia X-linked type 1 (SPG1) [MIM:303350]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Note=Defects in L1CAM may contribute to Hirschsprung disease by modifying the effects of Hirschsprung disease-associated genes to cause intestinal aganglionosis.
Defects in L1CAM are a cause of partial agenesis of the corpus callosum (ACCPX) [MIM:304100]. A syndrome characterized by partial corpus callosum agenesis, hypoplasia of inferior vermis and cerebellum, mental retardation, seizures and spasticity. Other features include microcephaly, unusual facies, and Hirschsprung disease in some patients.
Sequence similarities
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionP32004
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- antigen identified by monoclonal antibody R1 antibody
- CAML1 antibody
- CD 171 antibody
see all
Database links
- Entrez Gene: 3897 Human
- Omim: 308840 Human
- SwissProt: P32004 Human
- Unigene: 522818 Human
Anti-L1CAM antibody [UJ127] images:
Immunocytochemistry/ Immunofluorescence - L1CAM antibody [UJ127] (ab3200)
![Immunocytochemistry/ Immunofluorescence - L1CAM antibody [UJ127] (ab3200)](/ps/datasheet/Images/3/ab3200/ab3200_1.jpg)
ab3200 at a dilution of 1/1000, staining L1CAM (green; Alexa 488 secondary at 1/2000) on 30
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
Western blot - L1CAM antibody [UJ127] (ab3200)
![Western blot - L1CAM antibody [UJ127] (ab3200)](/ps/datasheet/images/3/ab3200/L1CAM-Primary-antibodies-ab3200-2.jpg)
Anti-L1CAM antibody [UJ127] (ab3200) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 230 kDa (why is the actual band size different from the predicted?)
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
L1CAM contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Protocols
References for Anti-L1CAM antibody [UJ127] (ab3200)
This product has been referenced in:
- Schlatter MCet al. CHL1 promotes Sema3A-induced growth cone collapse and neurite elaboration through a motif required for recruitment of ERM proteins to the plasma membrane. J Neurochem 104:731-44 (2008).Read more (PubMed: 17995939) »
- Grønborg Met al. Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol Cell Proteomics 5:157-71 (2006).Read more (PubMed: 16215274) »
See all 2 publications for this product
Publishing research using ab3200? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"