|
|
|
|
Immunocytochemistry/ Immunofluorescence - L1CAM antibody [UJ127] (ab3200)
![Immunocytochemistry/ Immunofluorescence - L1CAM antibody [UJ127] (ab3200)](/ps/datasheet/images/ab3200_1.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
L1CAM antibody [UJ127]
|
| |
See all L1CAM antibodies (10)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [UJ127] to L1CAM
|
|
Immunogen
|
Homogenous suspension of 16 week human fetal brain.
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
ab3200 is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin. It does not bind to pediatric or adult brain.
|
|
Tested applications
(see key)
|
ICC/IF, IHC-P, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IF: 1/500 - 1/1000.
IHC-P: Use at a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at 2µg/mg of lysate.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 200-220 kDa. It may also detect smaller cleavage fragments (please see Notes below).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
IMR-5 cells.
|
|
Cellular localization
|
Cell membrane; Single-pass type I membrane protein.
|
|
Research areas
|
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Liver
|
|
Relevance
|
The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
UJ127
|
|
Isotype
|
IgG1
|
|
Light chain type
|
kappa
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
0.200 mg/ml
|
|
Storage instructions
|
Store at +4°C. Do not freeze.
|
|
Notes
|
L1CAM can be detected between 200-220 kD. In brain samples it is typically seen at ~ 200 kD. When the protein is overexpressed in vitro it is often detected as a doublet with bands at 200 and 220 kD. The unglycosylated, unprocessed L1CAM is ~ 140-150 kDa. The protein has 21 putative N-glycosylation sites on the extracellular portion of the protein which, when they are all glycosylated, results in a detected MW of 200-220 kD depending upon how many residues are actually glycosylated. L1CAM can be cleaved by the metalloprotease ADAM10 resulting in fragments of 180 kD and 40 kD. L1CAM can also be cleaved by plasmin resulting in fragments of 140 kD and 80 kD. In theory, therefore, one could detect bands at ~220, 200, 180, 140, 80 and 40 kD.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this L1CAM antibody [UJ127] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for L1CAM antibody [UJ127] images, references, products related to ab3200 and other tools.
|
| |
L1CAM antibody [UJ127] images:
|
 Immunocytochemistry/ Immunofluorescence - L1CAM antibody [UJ127] (ab3200)
ab3200 at a dilution of 1/1000, staining L1CAM (green; Alexa 488 secondary at 1/2000) on 30µm coronal rat brain tissue sections in free floating IHC (see protocol link for detailed description). Images showing neuron body, cytoplasm and axon labeling: [A] neuron; 40x objective [B] neuron and axons; 40x objective and [C] punctate cytoplasmic labeling. Images coloured in Photoshop.
NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
|
| |
References for L1CAM antibody [UJ127] (ab3200)
|
|
This product has been used in: (two most recent references)
Schlatter MC et al. CHL1 promotes Sema3A-induced growth cone collapse and neurite elaboration through a motif required for recruitment of ERM proteins to the plasma membrane. J Neurochem 104:731-44 (2008). PubMed: 17995939
Grnborg M et al. Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach. Mol Cell Proteomics 5:157-71 (2006). PubMed: 16215274
If you publish research using ab3200 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to L1CAM
|
| |
L1CAM antibody [UJ127] - more information
|
Customer reviews (feedback) regarding L1CAM antibody [UJ127] |
Customer FAQs regarding L1CAM antibody [UJ127] |
Protocols for L1CAM antibody [UJ127] |
Price and availability of products related to L1CAM antibody [UJ127] |
| |
L1CAM antibody [UJ127] - related products:
|
|
|
| |
L1CAM antibody [UJ127] - other tools:
|
Contact Abcam with a Technical Enquiry about L1CAM antibody [UJ127] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunocytochemistry/ Immunofluorescence - L1CAM antibody [UJ127] (ab3200)](/index.html?datasheet=3200#ab3200_1.jpg)
