L1CAM protein (Tagged-His Tag) (ab91697)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
QLLVVGSPGPVPRLVLSDLHLLTQSQ VRVSWSPAEDHNAPIEKYDIEFEDK EMAPEKWYSLGKV PGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSET VVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAP QVQYRVQWRPQ GTRGPWQEQIVSDPFLVVSNTSTFVPY EIKVQAVNSQGKGPEPQVTIGYSG EDYPQAIPELEGIE ILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQR K HSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNG RGSGPASEFT FSTPEGVPGHPEALHLECQSNTSLLLRW QPPLSHNGVLTGYVLSYHPLDEG GKGQLSFNLRDPELR THNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTM AL SGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKAL GEEKGGASL SP
Our Abpromise guarantee covers the use of ab91697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Antigen identified by monoclonal antibody R1CAML1CD171
- CD171 antigenHSASHSAS1HydL1L1 cell adhesion moleculeL1-NCAML1camL1CAM_HUMANMASAMIC5N CAML1N-CAM-L1NCAM-L1NCAML1Nerve-growth factor-inducible large external glycoproteinNeural cell adhesion molecule L1NILEOTTHUMP00000025992S10SPG1
Defects in L1CAM are the cause of mental retardation-aphasia-shuffling gait-adducted thumbs syndrome (MASA) [MIM:303350]; also known as corpus callosum hypoplasia, psychomotor retardation, adducted thumbs, spastic paraparesis, and hydrocephalus or CRASH syndrome. MASA is an X-linked recessive syndrome with a highly variable clinical spectrum. Main clinical features include spasticity and hyperreflexia of lower limbs, shuffling gait, mental retardation, aphasia and adducted thumbs. The features of spasticity have been referred to as complicated spastic paraplegia type 1 (SPG1). Some patients manifest corpus callosum hypoplasia and hydrocephalus. Inter- and intrafamilial variability is very wide, such that patients with hydrocephalus, MASA, SPG1, and agenesis of corpus callosum can be present within the same family.
Defects in L1CAM are the cause of spastic paraplegia X-linked type 1 (SPG1) [MIM:303350]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Note=Defects in L1CAM may contribute to Hirschsprung disease by modifying the effects of Hirschsprung disease-associated genes to cause intestinal aganglionosis.
Defects in L1CAM are a cause of partial agenesis of the corpus callosum (ACCPX) [MIM:304100]. A syndrome characterized by partial corpus callosum agenesis, hypoplasia of inferior vermis and cerebellum, mental retardation, seizures and spasticity. Other features include microcephaly, unusual facies, and Hirschsprung disease in some patients.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
L1CAM protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for L1CAM protein (Tagged-His Tag) (ab91697)
ab91697 has not yet been referenced specifically in any publications.