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Anti-L3MBTL2 antibody (ab64422) at 1.25 µg/ml + Transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 79 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Diluted in 5% skim milk / PBS buffer, gel concentration 12%
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