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Western blot - L3MBTL2 antibody (ab64422)

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Product Name
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L3MBTL2 antibody
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See all L3MBTL2 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to L3MBTL2
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Immunogen
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Synthetic peptide: LLEDDPQGAR KISSEPVPGE IIAVRVKEEH LDVASPDKAS SPELPVSVEN, corresponding to a region within C terminal amino acids 649-698 of Human L3MBTL2 BLAST 'LLEDDPQGAR KISSEPVPGE IIAVRVKEEH LDVASPDKAS SPELPVSVEN ' with or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: Titre using peptide based assay 1/62500 WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 79 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Transfected 293T cell lysate
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> Other Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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L3MBTL2 is a putative Polycomb group (PcG) protein which contains 1 FCS-type zinc finger and 4 MBT repeats. These proteins are believed to cause a chromatin modification that leads to an inheritable repression in gene transcription. It is believed that its association with a chromatin remodelling complex may also contribute to prevent expression of genes that trigger the cell into mitosis.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this L3MBTL2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for L3MBTL2 antibody images, references, products related to ab64422 and other tools.
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L3MBTL2 antibody images:
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Western blot - L3MBTL2 antibody (ab64422)
L3MBTL2 antibody (ab64422) at 1.25 µg/ml + Transfected 293T cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 79 kDa Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Diluted in 5% skim milk / PBS buffer, gel concentration 12%
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Search PubMed (MEDLINE) for references to L3MBTL2
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L3MBTL2 antibody - more information
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Customer reviews (feedback) regarding L3MBTL2 antibody |
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L3MBTL2 antibody - related products:
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L3MBTL2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about L3MBTL2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"