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Western blot - L3MBTL2 antibody (ab64422)
Western blot - L3MBTL2 antibody (ab64422)
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Product Name 

L3MBTL2 antibody

 

See all L3MBTL2 antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to L3MBTL2

Immunogen 

Synthetic peptide: LLEDDPQGAR KISSEPVPGE IIAVRVKEEH LDVASPDKAS SPELPVSVEN, corresponding to a region within C terminal amino acids 649-698 of Human L3MBTL2
BLAST 'LLEDDPQGAR KISSEPVPGE IIAVRVKEEH LDVASPDKAS SPELPVSVEN ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Titre using peptide based assay 1/62500
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 79 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Transfected 293T cell lysate

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

Relevance 

L3MBTL2 is a putative Polycomb group (PcG) protein which contains 1 FCS-type zinc finger and 4 MBT repeats. These proteins are believed to cause a chromatin modification that leads to an inheritable repression in gene transcription. It is believed that its association with a chromatin remodelling complex may also contribute to prevent expression of genes that trigger the cell into mitosis.

 

Alternative names L3MBTL2 antibody (ab64422)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

83746    (Human)

Omim

    

611865    (Human)

SwissProt

    

Q969R5    (Human)

Unigene

    

517641    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this L3MBTL2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for L3MBTL2 antibody images, references, products related to ab64422 and other tools.

 

L3MBTL2 antibody images:

Product image  Western blot - L3MBTL2 antibody (ab64422)

Western blot - L3MBTL2 antibody (ab64422)

L3MBTL2 antibody (ab64422) at 1.25 µg/ml + Transfected 293T cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 79 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)


Diluted in 5% skim milk / PBS buffer, gel concentration 12%

 
 

Search PubMed (MEDLINE) for references to L3MBTL2

 

L3MBTL2 antibody - more information

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L3MBTL2 antibody - related products:

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Lysates

 

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Other L3MBTL2 Antibodies

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L3MBTL2 antibody - other tools:

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