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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-L3MBTL2 antibody
See all L3MBTL2 products (5) ...
Description
Rabbit polyclonal to L3MBTL2
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: LLEDDPQGAR KISSEPVPGE IIAVRVKEEH LDVASPDKAS SPELPVSVEN, corresponding to a region within C terminal amino acids 649-698 of Human L3MBTL2
LLEDDPQGAR KISSEPVPGE IIAVRVKEEH LDVASPDKAS SPELPVSVEN
Positive control
Transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Western blot - L3MBTL2 antibody (ab64422)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
ELISA: Titre using peptide based assay 1/62500
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 79 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'.
Sequence similarities
Contains 1 FCS-type zinc finger.
Contains 4 MBT repeats.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ969R5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 3bt like 2 protein antibody
- 3bt like protein antibody
- DKFZP761I141 antibody
see all
Database links
- Entrez Gene: 83746 Human
- Omim: 611865 Human
- SwissProt: Q969R5 Human
- Unigene: 517641 Human
Anti-L3MBTL2 antibody images:
Western blot - L3MBTL2 antibody (ab64422)
Anti-L3MBTL2 antibody (ab64422) at 1.25 µg/ml + Transfected 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 79 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Diluted in 5% skim milk / PBS buffer, gel concentration 12%
References for Anti-L3MBTL2 antibody (ab64422)
ab64422 has not yet been referenced specifically in any publications.
Publishing research using ab64422? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"