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Western blot - LACTB antibody (ab74723)
Western blot - LACTB antibody (ab74723)
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Product Name 

LACTB antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to LACTB

Immunogen 

A synthetic peptide corresponding to a region of Human LACTB

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA titre using peptide based assay: 1:12500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 61 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HepG2 cell lysate

Cellular localization 

Mitochondrion

Research areas 

Signal Transduction >> Metabolism >> Mitochondrial
Chromatin and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome

Relevance 

LACTB is a protein from the large 39S subunit of the mitochondrial ribosome (mitoribosome). It has some sequence similarity to prokaryotic beta-lactamases but most of the residues that are responsible for the beta-lactamase activity are not conserved between the two proteins. It is expressed predominantly in skeletal muscle. There are two named isoforms.

 

LACTB antibody (ab74723)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

114294    (Human)

Omim

    

608440    (Human)

SwissProt

    

P83111    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this LACTB antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for LACTB antibody images, references, products related to ab74723 and other tools.

 

LACTB antibody images:

Product image  Western blot - LACTB antibody (ab74723)

Western blot - LACTB antibody (ab74723)

LACTB antibody (ab74723) at 1 µg/ml + HepG2 cell lysate at 10 µg/ml

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 61 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to LACTB

 

LACTB antibody - more information

Customer reviews (feedback) regarding LACTB antibody

Customer FAQs regarding LACTB antibody

Protocols for LACTB antibody

Price and availability of products related to LACTB antibody

 

LACTB antibody - related products:

Products for Western blot

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Lysates

 

Compatible Secondaries for LACTB antibody

ab6564

  

Cy5 ® antibody

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HRP antibody

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Texas Red ® antibody

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Phycoerythrin antibody

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Agarose antibody

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10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

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Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

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LACTB antibody - other tools:

Contact Abcam with a Technical Enquiry about LACTB antibody

 

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