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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-LACTB antibody
Description
Rabbit polyclonal to LACTB
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
A synthetic peptide corresponding to a region of Human LACTB
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Mitochondrial
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
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Western blot - LACTB antibody (ab74723)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA titre using peptide based assay: 1:12500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 61 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
LACTB is a protein from the large 39S subunit of the mitochondrial ribosome (mitoribosome). It has some sequence similarity to prokaryotic beta-lactamases but most of the residues that are responsible for the beta-lactamase activity are not conserved between the two proteins. It is expressed predominantly in skeletal muscle. There are two named isoforms.
Cellular localization
Mitochondrion
Alternative names
- G24 antibody
- Lactamase, beta antibody
- Mitochondrial 39S ribosomal protein L56 antibody
see all
Database links
- Entrez Gene: 114294 Human
- Entrez Gene: 80907 Mouse
- Entrez Gene: 300803 Rat
- Omim: 608440 Human
- SwissProt: P83111 Human
- SwissProt: Q9EP89 Mouse
Anti-LACTB antibody images:
Western blot - LACTB antibody (ab74723)
Anti-LACTB antibody (ab74723) at 1 µg/ml + HepG2 cell lysate at 10 µg/ml
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 61 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
References for Anti-LACTB antibody (ab74723)
ab74723 has not yet been referenced specifically in any publications.
Publishing research using ab74723? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"