PE Anti-LAIR1 antibody [113] (ab95749)
Key features and details
- PE Armenian hamster monoclonal [113] to LAIR1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
PE Anti-LAIR1 antibody [113]
See all LAIR1 primary antibodies -
Description
PE Armenian hamster monoclonal [113] to LAIR1 -
Host species
Armenian hamster -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse LAIR1. ARHO12 clone stably expressing Mouse LAIR1 fused to a C terminal MYC tag.
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Positive control
- C57BL/6 splenocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
113 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 0.125-0.25µg for 105-8 cells.
A 100 µl volume is recommended for staining the cell sample. |
Notes |
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Flow Cyt
Use 0.125-0.25µg for 105-8 cells. A 100 µl volume is recommended for staining the cell sample. |
Target
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Function
Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells. -
Tissue specificity
Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Developmental stage
Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation. -
Domain
ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation. -
Post-translational
modificationsPhosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
N-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 52855 Mouse
- SwissProt: Q8BG84 Mouse
- Unigene: 290880 Mouse
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Alternative names
- 5133400O11Rik antibody
- CD305 antibody
- D7Bwg0421e antibody
see all
Images
Datasheets and documents
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Datasheet download
References (1)
ab95749 has been referenced in 1 publication.
- Lebbink RJ et al. Mouse leukocyte-associated Ig-like receptor-1 (mLAIR-1) functions as an inhibitory collagen-binding receptor on immune cells. Int Immunol 19:1011-9 (2007). Flow Cyt . PubMed: 17702987