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Products:Immunology >> Immunoglobulins >> Receptors
Overview
Product name
Anti-LAIR1 antibody [lc12]
See all LAIR1 products (8) ...
Description
Mouse monoclonal [lc12] to LAIR1
Tested applications
ELISA, Flow Cyt, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant extracellular domain (amino acids 22-125) of human LAIR1
Epitope
Extracellular domain of LAIR1
Positive control
Human peripheral blood mononuclear cells or natural killer cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
lc12
Myeloma
Sp2/0
Isotype
IgG1
Light chain type
kappa
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab14826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 32 kDa.
IHC-P: Use at an assay dependent dilution.
Target
Function
Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, downregulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
Tissue specificity
Expressed on the majority of peripheral mononuclear cells, including natural killer (NK) cells, T-cells, B-cells, monocytes, and dendritic cells. Highly expressed in naive T-cells and B-cells but no expression on germinal center B-cells. Abnormally low expression in naive B-cells from HIV-1 infected patients. Very low expression in NK cells from a patient with chronic active Epstein-Barr virus infection.
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Developmental stage
Complete loss of expression when naive B-cells proliferates and differentiates into Ig-producing plasma cells under in vitro stimulation.
Domain
ITIM (immunoreceptor tyrosine-based inhibitor motif) motif is a cytoplasmic motif present in 2 copies in the intracellular part of LAIR1. When phosphorylated, ITIM motif can bind the SH2 domain of several SH2-containing phosphatases, leading to down-regulation of cell activation.
Post-translational
modifications
Phosphorylation at Tyr-251 and Tyr-281 activates it. May be phosphorylated by LCK.
N-glycosylated.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ6GTX8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 5133400O11Rik antibody
- CD305 antibody
- D7Bwg0421e antibody
see all
Database links
- Entrez Gene: 3903 Human
- Omim: 602992 Human
- SwissProt: Q6GTX8 Human
- Unigene: 572535 Human
References for Anti-LAIR1 antibody [lc12] (ab14826)
ab14826 has not yet been referenced specifically in any publications.
Publishing research using ab14826? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"